| 张霞,杨如俊,张新,等.RSPCM对受不同剂量照射小鼠的疗效观察[J].中华放射医学与防护杂志,2000,20(4):235-238.ZHANG Xia,YANG Rujun,ZHANG Xin,et al.Radioprotective effect of RSP CM on mice irradiated with different doses[J].Chin J Radiol Med Prot,2000,20(4):235-238 |
| RSPCM对受不同剂量照射小鼠的疗效观察 |
| Radioprotective effect of RSP CM on mice irradiated with different doses |
| 投稿时间：1999-10-29 |
| DOI： |
| 中文关键词: 细胞因子 辐射损伤 小鼠 |
| 英文关键词:Cytokines Radiation injury Mice |
| 基金项目:国家自然科学基金资助项目(39600077) |
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| 摘要点击次数: 3682 |
| 全文下载次数: 1999 |
| 中文摘要: |
| 目的 观察造血生长因子对受照射小鼠的防治作用。方法 用RSPCM和LP3-CM来源的集落刺激因子治疗受不同剂量60Coγ射线照射的ICR小鼠。通过观察30d存活率、死亡动物平均存活天数、保护系数及部分动物外周血白细胞计数、骨髓有核细胞计数和粒单系祖细胞集落培养, 研究GMCSF对受照射小鼠重建造血的疗效及量效、时效关系。结果 RSPCM能显着提高7.5Gy照射小鼠的存活率, 而LP3-CM效果不显。提高剂量到8.0Gy, 只有RSPCM治疗组有效。外周血WBC和BMC计数及祖细胞集落培养也显示治疗组优于预防组。8.5Gy照射小鼠30d内全部死亡, 表明GMCSF对大剂量照射小鼠疗效差。但从保护系数看, 各实验组均有效, 且存在量效、时效关系。结论 GMCSF对受照射小鼠的骨髓损伤有促进造血恢复的作用, 且存在量效、时效关系, 单纯应用MCSF则无效。 |
| 英文摘要: |
| Objective To investigate the radioprotective effects of cytokines on hematopoietic impairment of irradiated mice. Methods Using RSP-CMand LP3-CMrespectively originated GM-CSFand G-CSFto treat ICRmice irradiated with different doses of 60Co γ-rays.The 30-day survival rate of mice, the mean survival days of dead mice were determined and the numbers of peripheral white blood cells and BMCof part of the mice were counted.At the same time, GMclonogenic activity of BMwas assayed. Results RSP-CMcould effectively raise 30-day survival rate of mice irradiated with 7.5 Gy.However, LP3-CMhad no obvious effect.Judging from the comparative survival ratio, only the RSP-CMtreated group showed protective effect on the 8.0 Gy -irradiated mice.The 8.5 Gy-irradiated mice all died within30 days, indicating that GM-CSFhad weak effect on higher dose-irradiated mice. Conclusion GM-CSFcan stimulate the hematopoietic system of irradiated mice, and has dose-effect and time-effect relations.M-CSFused singly has no obvious effect. |
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