| 孙义敏,刘树铮.X射线全身照射对小鼠腹腔巨噬细胞NO产生的影响[J].中华放射医学与防护杂志,2000,20(4):232-234.SUN Yimin,LIU Shuzheng.Effect of whole body X irradiation on production of nitric oxide in peritoneal macrophages of mice[J].Chin J Radiol Med Prot,2000,20(4):232-234 |
| X射线全身照射对小鼠腹腔巨噬细胞NO产生的影响 |
| Effect of whole body X irradiation on production of nitric oxide in peritoneal macrophages of mice |
| 投稿时间：1999-10-29 |
| DOI： |
| 中文关键词: 电离辐射 巨噬细胞 iNOS NO |
| 英文关键词:Irradiation Macrophages Induced nitric oxide synthase Nitric oxide |
| 基金项目:国家自然科学基金资助项目(39570188) |
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| 中文摘要: |
| 目的 观察75mGy、20GyX射线全身照射后不同时间, 小鼠腹腔巨噬细胞NO产生的时程变化及不同剂量照射后24h时NO、iNOS的含量变化。方法 分光光度法及免疫组织化学方法。结果 iNOS和NO的产生与剂量呈正相关。20Gy照射后, NO从6h到48h随时间推移产生量逐渐增加, 48h达峰值, 为对照的38倍, 而后回降。结论 电离辐射可刺激巨噬细胞合成iNOS及产生NO。 |
| 英文摘要: |
| Objective To disclose the changes of nitric oxide (NO) in murine peritoneal macrophages at different time intervals after whole body irradiation (WBI) with 75 mGy and 2.0 Gy X-rays and the changes of NOand induced nitric oxide synthase (iNOS)24 hours after WBIwith 0.05-6.0 Gy X-rays. Methods Immunohistochemistry and spectophotometry were used. Results The expression of iNOSand the production of NOincreased in a dose-dependent manner after irradiation.The production of NOincreased from 6h to 48h and reached its peak at 48h, which was 3.8-fold of sham-irradiated controls. Conclusion Ionizing radiation stimulates expression of iNOSand production of NOin peritoneal macrophages of mice. |
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