| 刘胜学,曹佳,安辉,周紫恒,孙华明.用多重PCR分析60Coγ射线引起人白血病细胞HPRT基因突变[J].中华放射医学与防护杂志,2000,20(4):225-227 |
| 用多重PCR分析60Coγ射线引起人白血病细胞HPRT基因突变 |
| Molecular analysis of 60Co gamma-ray-induced mutation at HPRT locus in human promyelocytic leukemia cells |
| 投稿时间：1999-08-02 |
| DOI： |
| 中文关键词: HPRT基因 60Coγ射线 致突变作用 多重PCR |
| 英文关键词:HPRT gene 60Co γ-rays Mutagenesis Multiplex PCR |
| 基金项目:国家自然科学基金资助项目(39970650) |
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| 中文摘要: |
| 目的 探讨60Coγ射线诱导人HPRT基因突变的分子图谱和发生机理, 以及与辐射抗肿瘤的关系。方法 采用单细胞克隆培养、双向筛选计数、多重PCR扩增和电泳分析等方法。结果 ①随着辐射剂量的增加, 细胞接种存活率下降, 突变频率升高。②自发突变中92.3%是点突变, 而γ射线诱发突变主要由缺失与点突变两部分组成(两者分别为617%和38.3%).③缺失突变可以发生于HPRT基因的每个外显子, 但诱发突变中绝大多数是多个外显子的连锁缺失(97.9%).结论 γ射线诱发HPRT基因突变图谱与自发突变不一样, 其易发生较大遗传结构改变的特点与作用机理有关。 |
| 英文摘要: |
| Objective To explore the spectra and mechanism of human HPRTgene mutation induced by 60Co γ-rays and its relation with antitumor effect of radiation.Methods Single cell clone culturing, twoway screening count, multiplex PCRamplification and electrophoresis technique were used. Results (1)When doses were increasing, cell plating efficiency reduced and mutation frequency increased.(2)The most frequent spontaneous mutations were point mutation (92.3%) and gammarayinduced mutation, including mainly partial deletion and point mutation(61.7% and 38.3%, respectively).(3)There were deletion mutations in all 9 exons of HPRTgene and the most of gammarayinduced mutations were chain deletion with multiplex exons (97.9%).Conclusion The spectra of spontaneous and gammarayinduced mutants were different.The bigger changes in genetic structure are related to the antitumor mechanism of radiation. |
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