中华放射医学与防护杂志

李雨,赵芳,郑秀龙,等.WT对辐射致DNA损伤修复的影响[J].中华放射医学与防护杂志,2000,20(3):176-178.LI Yu,ZHAO Fang,ZHENG Xiulong,et al.Wortmannin inhibits repair of DNA double strand breaks induced by radiation.[J].Chin J Radiol Med Prot,2000,20(3):176-178

WT对辐射致DNA损伤修复的影响

Wortmannin inhibits repair of DNA double strand breaks induced by radiation.

投稿时间：1999-12-08

DOI：

中文关键词: Wortmannin 放射增敏 DNA双链断裂

英文关键词:Wortmannin Radiosensitization DNA double strand break

基金项目:

作者	单位
李雨	200433 上海, 第二军医大学放射医学教研室
赵芳	200433 上海, 第二军医大学放射医学教研室
郑秀龙	200433 上海, 第二军医大学放射医学教研室
罗成基	第三军医大学防原教研室
蔡建明	200433 上海, 第二军医大学放射医学教研室

摘要点击次数: 3174

全文下载次数: 1999

中文摘要:

目的研究磷脂酰肌醇3-激酶的特异性抑制剂Wortmannin(WT)对小鼠胸腺细胞DNA辐射损伤修复的影响, 以探索WT放射增敏作用机制。方法以50μmol/LWT作用小鼠胸腺细胞, 以不同剂量γ射线照射, 照射后不同时间制取细胞DNA, 用琼脂糖凝胶电泳方法检测DNA双链断裂(dsb).结果 WT单独对受照射细胞dsb产额无明显影响, 抑制照射后dsb修复。结论 WT的放射增敏剂作用与其抑制照射后dsb修复有关。

英文摘要:

Objective To study the effect of the specific inhibitor Wortmannin(WT)on repair of radiation damage of thymocyte DNA in mice. Method The cells were exposed to WT or DMSO(solvent)for 1 h, and then irradiated at the indicated doses.After indicated times post irradiation, the cells were washed to remove the WT, their DNA was made out, then DNA damage and repair were detected by electrophoresis. Results After 10 Gy and 20 Gy γ irradiation, dsb level was higher in WT group than in control group.It was more evident at 1-3 hrs after irradiation. Conclusion The radiosensitizing effect of WT is related to its inhibition of dsb repair after irradiation.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭