| 曹建平,马岛秀行,山口千鹤.应用脉冲电场凝胶电泳分析X射线诱发人骨肉瘤细胞DNA双链断裂与损伤修复效应[J].中华放射医学与防护杂志,2000,20(3):159-163 |
| 应用脉冲电场凝胶电泳分析X射线诱发人骨肉瘤细胞DNA双链断裂与损伤修复效应 |
| PFGE analysis of DNA double-strand breaks and DNA repair process in human osteosarcoma cells irra-diated by X-rays |
| 投稿时间：2000-02-17 |
| DOI： |
| 中文关键词: 骨肉瘤 X射线 脉冲电场凝胶电泳 DNA双链断裂 DNA修复 |
| 英文关键词:Osteosarcoma Xray irradiation Pulsedfield gel electrophoresis DNA doublestrand breaks DNA repair |
| 基金项目:日本科技厅研究基金资助项目(497009) |
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| 中文摘要: |
| 目的 研究电离辐射诱发人骨肉瘤肿瘤细胞DNA双链断裂与辐射损伤修复效应, 观察辐射损伤、损伤修复效应与肿瘤细胞辐射敏感性之间的关系。方法 选用强制均匀电场电泳, 分别测定经不同剂量X射线照射和相同剂量照射后培养不同时间, 人骨肉瘤Rho0和143.B肿瘤细胞株DNA双链断裂。结果 (1)X射线诱发人骨肉瘤肿瘤细胞的DNA双链断裂与辐射剂量呈线性正比关系; (2)培养后的人骨肉瘤肿瘤细胞对辐射诱发的DNA双链断裂具有一定修复能力; (3)Rho0比143.B细胞株具有更高的辐射敏感性; (4)脉冲电场凝胶电泳技术是分析人肿瘤细胞DNA双链断裂的敏感方法。结论 脉冲电场凝胶电泳是分析人肿瘤细胞DNA双链断裂的敏感方法; 电离辐射诱发人骨肉瘤细胞DNA双链断裂与损伤修复效应和肿瘤细胞的辐射敏感性有密切关系。 |
| 英文摘要: |
| Objective To study the induction of DNA doublestrand breaks (DSBs) in human osteosarcoma cells irradiated by Xray, the DNA DSBs repair process and the tumour cell radiosensitivity. Methods Two cell lines of human osteosarcoma, Rho0 and 14.3B were used.Initial DNA damage of DSBs by X-ray irradiation was measured using clamped homogeneous electrical field (CHEF) electrophoresis. Results X-rayinduced DNA DSBs of human osteosarcoma cells after CHEFelectrophoresis increased linearly with the irradiation dose between 0 and 50 Gy.The repair of DNA DSBs in human osteosarcoma cells increased with the postirradiation incubation time.In contrast to 14.3B cell line at the same dose point, much more DNA DSBs were induced in Rho0 cell line after X-ray irradiation. Conclusion CHEF pulsedfield gel electrophoresis (PEGE) is a sensitive method for the determination of radiationinduced DNA DSBs in high molecular weight DNA of human osteosarcoma cells.Radiationinduced DNA DSBs of osteosarcoma increase with the dose in a linear manner.after incubation, both Rho0 cell line and 143.B cell line can repair the DNA DSBs.Between two cell lines of human osteosarcoma, Rho0 and 14.3B, Rho0 cell line is more sensitive to ionizing radiation than 14.3B line. |
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