| 田琼,张绍章,张发科.GM-CSF和rhBMP-2m对小鼠急性放射损伤治疗作用的对比研究[J].中华放射医学与防护杂志,2000,20(3):157-158.TIAN Qiong,ZHANG Shaozhang,ZHANG Fake.Comparative study of therapeutic effect of GM-CSF and rhBMP-2m on acute radiation injury in mice[J].Chin J Radiol Med Prot,2000,20(3):157-158 |
| GM-CSF和rhBMP-2m对小鼠急性放射损伤治疗作用的对比研究 |
| Comparative study of therapeutic effect of GM-CSF and rhBMP-2m on acute radiation injury in mice |
| 投稿时间：2000-02-14 |
| DOI： |
| 中文关键词: 粒巨细胞集落刺激因子 重组人骨形成蛋白-2 放射损伤 小鼠 |
| 英文关键词:GMCSF rhBMP-2m Acute radiation sickness Mice |
| 基金项目:国家自然科学基金项目(39970231) |
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| 摘要点击次数: 2971 |
| 全文下载次数: 2020 |
| 中文摘要: |
| 目的 研究和比较重组人骨形成蛋白(rhBMP2m)、GM-CSF对照射后小鼠造血损伤的修复作用。方法 rhBMP2m用分子生物学方法由大肠杆菌中获得; 该实验中rhBMP2m对照射后动物的影响, 通过观察动物的30天活存率表示。结果 照射对照组小鼠的30天活存率为0, 平均活存天数为(9.1±2.2)d;GMSCF治疗组小鼠的30天活存率为20.0%, 平均活存天数为(11.5±1.9)d, rhBMP2m治疗组小鼠的30天活存数为4, 活存率为40%, 平均活存天数为(13.2±6.1)d.结果显示GMCSF和rhBMP2m均能提高受照小鼠的活存率, 并延长活存时间, 而且后者优于前者(P<0.05).结论 rhBMP2m对小鼠急性放射损伤具有治疗作用, 初步观察其优于GMCSF。 |
| 英文摘要: |
| Objective To investigate the effect of bone morphogenetic proteins-2m (rhBMP-2m)on hematopoietic type of acute radiation sickness in mice. Methods rhBMP-2m was obtained from E.coli by molecular biological method.In this study the effect of rhBMP-2m on hematopoiesis was detected post irradiation and the 30 days survival rates were observed. Results Mice of the irradiation control group died in 30 days.The survival rate of rhBMP-2m treated mice was higher than that of the GMCSF treated ones ( P <0.05).The effect of thBMP-2m on the survival of mice after 7.0 Gy irradiation was that 40% of mice survived after i.p.injection with 0.25 mg, but in GMCSF group i.p.injection of 300 ng was needed. Conclusion These data support our hypothesis that rhBMP-2m can be used to treat the hematopoietic type of acute radiation sickness. |
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