| 孟祥兵,宋宜,毛建平,巩波,董燕,刘斌,孙志贤.ATM参与辐射损伤细胞学反应的研究[J].中华放射医学与防护杂志,2000,20(3):149-152 |
| ATM参与辐射损伤细胞学反应的研究 |
| Participation of ATM in cellular response to DNA damage induced by ionizing radiation |
| 投稿时间：1999-12-08 |
| DOI： |
| 中文关键词: ATM PI3K 结构域 长片段PCR 酵母双杂交 |
| 英文关键词:ATM P13K Domain LDPCR Yeast twohybrid system |
| 基金项目:国家自然科学基金资助项目(39700038)全军医药卫生九五重点基金资助项目(9605007) |
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| 中文摘要: |
| 目的 克隆ATM全长cDNA及含特异功能域的cDNA片段, 寻找与ATM相互作用的蛋白, 分析ATM在DNA损伤监视中的分子机理。方法 利用长片段PCR扩增法, 从人外周血来源的cDNA库中扩增ATMcDNA;利用酵母双杂交系统筛选人外周血来源的cDNA库中与ATM相互作用的蛋白。结果 经重叠PCR扩增到ATM全长cDNA, 并筛选到数条与ATMPI3K激酶区相互作用的cDNA, 并对其序列进行分析。结论 ATM通过与多种蛋白相互作用, 传递DNA损伤信号。 |
| 英文摘要: |
| Objective To clone ATM full length cDNA and cDNA fragments containing some functional domains and to identify proteins that interact with ATM and mediate DNA damage signal transduction in cellular response to DNA damage. Methods ATM cDNA was amplified from Marthom TM Ready cDNA kit of human leukocytes by LDPCR.ATMinteracting proteins were screened by yeast two hybrid system. Results ATM fulllength cDNA and cDNA fragments containing PI3K kinase domain, leucine zipper and proline rich region were amplified from human cDNAs.Several candidate clones that interacted with ATM PI3K domain were identified. Conclusion ATM mediates DNA damage signal transduction by interacting with many proteins. |
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