| 吴玮,杨如俊,孔宪涛,张玲珍,李伯龙,蔡建明.苯甲酸雌二醇对辐射诱导免疫细胞凋亡的影响[J].中华放射医学与防护杂志,2000,20(2):98-101 |
| 苯甲酸雌二醇对辐射诱导免疫细胞凋亡的影响 |
| Effects of estradiol on radiation-induced apoptosis in immunocytes of mouse |
| 投稿时间：1999-03-20 |
| DOI： |
| 中文关键词: 苯甲酸雌二醇 辐射损伤 细胞凋亡 免疫细胞 |
| 英文关键词:Radiation injury Apoptosis Immunocytes Estradiol |
| 基金项目: |
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| 中文摘要: |
| 目的 观察苯甲酸雌二醇对60Coγ射线引起的小鼠胸腺、脾脏细胞凋亡率的影响及脾脏T、B淋巴细胞表面分子表达的改变, 探讨其对小鼠免疫细胞凋亡的调节作用及其作用机理。方法 采用60Coγ射线4Gy单次全身照射, 用DNA琼脂糖凝胶电泳、PI染色及双荧光流式细胞术研究小鼠免疫细胞凋亡, 细胞表面CD95、CD25、CD69的表达变化规律。结果 注射照射组各细胞亚群凋亡率较单纯照射组明显降低, 照射后4h未出现照射组所呈现的CD95高表达。射线使脾脏细胞CD25和CD69表达升高, 而注射照射组未观察到CD25和CD69的高表达。结论 苯甲酸雌二醇可阻断免疫细胞的激活进而阻断Fas(CD95)系统介导的细胞凋亡途径。 |
| 英文摘要: |
| Objective To assess the effects of estradiol on 60 Coγ-radiation induced apoptosis of splenic lymphocytes and thymocytes,and surface molecule expression of splenic lymphocytes. Methods Mice were whole body irradiated with 4.0 Gy γ-rays. By flow cytometry and electrophoretic analysis of DNA, the changes in apoptosis of mouse immunocytes were determined. The splenic lymphocytes were analyzed by flow cytometry with fluorescent monoclonal antibodies. Results 10 days after administration of estradiol, the characteristic DNA ladder in mice 8h after irradiation was minor than in mice without estradiol administration,indicating that the apoptotic rate reduced on flow cytometry. CD4+ T cells,CD8+ T cells and IgM+ B cells upregulated Fas,CD25 and CD69 expression,but did not so in the estradiol treated mice. Conclusion Estradiol can block CD25,CD69 and Fas overexpression,thereby inhibiting Fas mediated apoptosis inducedby γ-irradiation. |
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