| 彭瑞云,王德文,熊呈琦,高亚兵,李延平,杨红,崔玉芳.γ射线全身照射后小鼠骨髓造血细胞IL-3基因表达的原位PCR检测及其意义[J].中华放射医学与防护杂志,2000,20(2):91-93 |
| γ射线全身照射后小鼠骨髓造血细胞IL-3基因表达的原位PCR检测及其意义 |
| In situ PCR detection and significance of IL-3 gene expression in irradiated hematopoietic cells of mouse bone marrow |
| 投稿时间：1998-07-21 |
| DOI： |
| 中文关键词: 辐射 骨髓 IL-3, 原位PCR |
| 英文关键词:Radiation Bone marrow Interlenkin-3 In situ PCR |
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| 中文摘要: |
| 目的 探讨内源性IL-3基因表达在骨髓辐射损伤后修复中的作用。方法 5.5Gy<60Coγ射线全身照射78只LACA小鼠, 于照射后4周内分批活杀, 将骨髓进行HE染色, 并进行内源性IL-3基因表达的免疫组化、原位杂交和原位反转录PCR检测。结果 照射后4周内小鼠骨髓出现明显损伤及损伤后重建现象。免疫组化观察IL-3于修复期造血细胞浆内明显增多, 尤以照后21天其量达高峰, 而原位杂交则显示其mRNA于照射后10～21天为弱阳性, 尤以照射后15天明显。原位反转录PCR显示IL-3mRNA于修复期造血细胞浆内明显增多, 尤以照射后10～15天为甚。结论 原位反转录PCR能客观反映IL-3基因表达在骨髓辐射损伤后的变化规律, 且内源性IL-3基因表达在骨髓辐射损伤后造血功能重建中可能起明显促进作用。 |
| 英文摘要: |
| Objective To study the significance of endogenous interleukin 3(IL-3) gene expression in repair of irradiated mouse bone marrow. Methods Seventy-eight LACA mice were subjected to total body irradiation with 60 Co γ-rays and were sacrificed within 4 weeks after irradiation.The bone marrow histopathological sections were stained with HE, and the expression of endogenous IL-3 gene was detected by means of immunocytochemistry, in situ hybridization(ISH) and in situ reverse transcription PCR(IS RT-PCR). Results Obvious injury of bone marrow occurred after irradiation and then recovered within 4 weeks.IL-3 protein was obviously increased in the cytoplasma of recovering hematopoietic cells(HCs),especially on day 21 after irradiation,while its mRNA was poorly positive by ISH on days 10-21,especially day 15.IS RT-PCR showed that IL-3 mRNA was strongly positive in recovering HCs cytoplasma,especially on days 10 to 15. Conclusion In situ RT-PCR can objectively reflect the regulation of IL-3 gene expression in bone marrow after irradiation,and the expression of endogenous IL-3 gene may play an important role in hematopoietic reconstruction of irradiated bone marrow. |
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