| 苑宾,李梁,薛文成,孙建民,王宝勤.裂变中子诱导小鼠胸腺细胞凋亡的剂量效应[J].中华放射医学与防护杂志,2000,20(2):87-90 |
| 裂变中子诱导小鼠胸腺细胞凋亡的剂量效应 |
| Dose-effect relationship of apoptosis induced by fission-neutron in murine thymocytes |
| 投稿时间：1999-02-02 |
| DOI： |
| 中文关键词: 裂变中子 γ射线 胸腺细胞 细胞凋亡 RBE |
| 英文关键词:Fission neutrons γ-rays Thymocytes Apoptosis RBE |
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| 中文摘要: |
| 目的 研究核反应堆裂变中子诱导小鼠胸腺细胞凋亡的剂量效应, 并与60Coγ射线的效应相比较, 探讨两种辐射诱导细胞凋亡的异同。方法 用光镜和电镜形态学观察与DNA琼脂糖凝胶电泳方法对细胞凋亡进行定性研究;用流式细胞术(FCM)和二苯胺(DPA)法对其进行定量研究。结果 小鼠经裂变中子0.5～5.0Gy照射后6h, 可检测到胸腺细胞的DNAladder, 光镜及电镜下可观察到胸腺细胞典型的凋亡形态, 且其凋亡比例随着照射剂量的增高而增加;γ射线1.0～3.0Gy照射后6h, 也都检测到胸腺细胞的DNAladder, 其凋亡比例随剂量增高而增加, 2.0Gy左右达到峰值, 剂量再高, 其凋亡比例不再增加。结论 0.5～5.0Gy的裂变中子或1.0～3.0Gy的γ射线照射小鼠, 均可引起胸腺细胞发生凋亡, 且随剂量的变化规律相似;但裂变中子诱导小鼠胸腺细胞凋亡的效应高于γ射线, 其RBE值约为2.09(FCM法)或2.37(DPA法).表明中子对小鼠免疫组织的损伤在照射后几小时就比γ射线重, 这可能是后期免疫组织损伤较重的基础。 |
| 英文摘要: |
| Objective To investigate the effectiveness of high LET fissionneutron to induce apoptosis in murine thymocytes and to compare it with that of low LET 60 Co γ ray. Methods Apoptosis induction was studied qualitatively by light and transmission electron microscopy and DNA gel electrophoresis,also quantitatively by flow cytometry(FCM) and diphenylamine (DPA)methods. Results DNA ladders of murine thymocytes were detectable,the typical apoptosis of thymocytes could be observed morphologically by means of light and electron microscopy at 6 h after fissionneutron irradiation with doses ranging from 0.5 to 5.0 Gy,meanwhile the percentages of apoptosis increased with increasing doses.After exposure to γ-rays with doses ranging from 1.0 to 30 Gy,the expermiental results were similar to those from neutron radiation.The incidence of apoptosis peaked at about 20 Gy,the percentages did not increase further when doses increased. Conclusion Apoptosis of murine thymocytes can be induced when mice are exposed to either fission-neutron (0.5-5.0 Gy) or to γ -ray (1-30 Gy).Although the relationship between apoptosis and radiation doses is similar,the percentage of apoptosis induced by neutron irradiation is higher than that induced by γ -irradiation.The RBE values of fission-neutron for inducing apoptosis murine thymocytes are 2.09 (by FCM method) and 2.37 (by DPA method),respectively.These results also suggest that fission-neutron-induced murine immune tissue is more severe than that induced by γ -rays at several hours post-irradiation and this might be the basis for heavy damage to immune tissues induced by fission-neutron-irradiation in later period. |
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