| 田野,包仕尧,刘春风,殷蔚伯.大鼠大脑照射后海马区细胞凋亡与病理形态学变化的研究[J].中华放射医学与防护杂志,2000,20(2):84-86 |
| 大鼠大脑照射后海马区细胞凋亡与病理形态学变化的研究 |
| Apoptosis and morphological changes in hippocampus of rat brain after hemisphere irradiation |
| 投稿时间：1999-04-13 |
| DOI： |
| 中文关键词: 电离辐射 海马 细胞凋亡 bcl-2, 形态学改变 |
| 英文关键词:Ionizing radiation Hippocampus Apoptosis bcl-2 Morphological changes |
| 基金项目:国家国防科工委项目(6920062-14) |
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| 摘要点击次数: 3081 |
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| 中文摘要: |
| 目的 了解大鼠大脑受电离辐射后早期海马区细胞凋亡、bcl-2表达和病理形态学变化的情况。方法 用流式细胞仪测定凋亡细胞的比例与bcl-2蛋白的含量, 用电镜和光镜分别作形态学变化的观察。结果 受20Gy照射后第1天起海马中就出现了凋亡细胞并逐步增加, 至照射后3个月时恢复正常, 而bcl-2蛋白的含量则呈相反的改变。在30Gy照射后3个月组中有坏死灶的存在, 而其他组别中可以看到血管、胶质细胞和神经元等组织的形态学异常。结论 大鼠大脑受辐射后早期海马区可发生神经细胞凋亡、bcl-2表达的下降, 以及各组织成分的病理形态学改变, 这些变化的程度与照射剂量和观测时间有关。 |
| 英文摘要: |
| Objective To study the apoptosis,the expression of bc1-2 and the morphological changes of various tissue components in the rat hippocampus after hemisphere irradiation. Methods The DNA contents and the quantities of bc1-2 protein were analyzed by flow cytometry.The histological sections were examined by electron and light microscopy.Results The apoptosis of hippocampal cells in the irradiated brain increased in most of the rats,the expression of bc1-2 decreased in the meantime,and those changes were significant only in the groups irradiated within 1 month.Necrosis was observed in some of the 30 Gy-3-month-period group. Different levels of morphological changes in blood vessels,neuroglia cells and neurons were found in rats of all other groups. Conclusion After hemisphere irradiation,apoptosis increases,bc1-2 protein decreases,and various components in the hippocampus change.These data suggest a possible association between the damage of various componens and development of radiation-induced brain toxicity. |
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