| 江国春,袁丽珍,魏康,郭学敏,张雪峰.HT1080R细胞的辐射抗性与细胞周期关系初步研究[J].中华放射医学与防护杂志,2000,20(2):80-83 |
| HT1080R细胞的辐射抗性与细胞周期关系初步研究 |
| Radioresistance and cell cycle distribution of HT1080R cells after γ-ray irradiation |
| 投稿时间：1999-09-10 |
| DOI： |
| 中文关键词: 辐射敏感性 细胞周期 p53 |
| 英文关键词:Radiosensitivity Cell cycle p53 |
| 基金项目: |
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| 中文摘要: |
| 目的 研究HT1080R的辐射抗性及其照射前后细胞周期分布, 探讨其辐射抗性的分子机理。方法 克隆形成比较HT1080与HT1080R照射后细胞存活率, 流式细胞术研究细胞周期分布, PCR-SSCP和Western杂交测定p53外显子的基因状态及其表达状况。结果 HT1080R的辐射敏感性降低, 具有明显的辐射抗性。照射后具有G2期阻滞现象, 而其p53基因5个外显子正常。结论 经γ射线照射HT1080R细胞周期改变与其亲本细胞明显不同, 出现明显的G2期阻滞, 提示HT1080R辐射抗性的获得可能与其细胞周期G2期阻滞有关, PCR-SSCP和Western杂交研究表明HT1080R细胞辐射抗性不是由于p53基因功能异常所致。 |
| 英文摘要: |
| Objective To investigate radioresistance and cell cycle distribution of HT1080R cells after γ ray irradiation. Methods By comparison of the survival fraction between HT1080R and HT1080 using cloning assay,cell cycle distribution and gene status were investigated by FCM and PCR-SSCP,respectively. Results HT1080R was more radioresistant than its parental cell line HT1080,and after exposure to 4 Gy and 6 Gy γ-rays showed G 2 phase arrest.PCR-SSCP showed that the expression of p53 gene was normal. Conclusion G2 arrest can be found after 4 Gy and 8 Gy γ ray irradiation in HT1080R cell line,which is more radioresistant than its parental cell line HT1080,suggesting that the acquirement of radioresistance may be related to its G 2 arrest.Because of its normal gene status and expression,radioresistance of HT1080R cannot be explained by abnormal function of p53 gene. |
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