| 黄定德,程绍钧,罗成基,粟永萍.放烧复合伤小鼠骨髓细胞IL-3R与造血调控的研究[J].中华放射医学与防护杂志,2000,20(1):36-39 |
| 放烧复合伤小鼠骨髓细胞IL-3R与造血调控的研究 |
| Relationship between IL-3R of BMNC and hematopoietic regulation in combined irradiation burn injury in mice |
| 投稿时间：1999-03-05 |
| DOI： |
| 中文关键词: 放射损伤 烧伤 放烧复合伤 造血调控 白细胞介素3受体 骨髓有核细胞 小鼠 |
| 英文关键词:Irradiation Burn Combined irradiation burn injury Hematopoietic regulation Interleukin 3 receptor Bone marrow Nucleated cells Mice |
| 基金项目:军队医药卫生“九五”指令性攻关项目(96L045) |
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| 中文摘要: |
| 目的 研究放烧复合伤小鼠骨髓有核细胞(BMNC)IL-3R的蛋白表达及功能的变化, 探讨放烧复合伤后IL-3R在造血调控中的作用。方法 小鼠随机分为正常(N)、放射损伤(R)、烧伤(B)及放烧复合伤(C)共4组, 分别于伤后1、3、7和14d观察BMNCIL-3Rα阳性率、平均含量及功能的变化, 及其与BMNC计数的相关性。结果 ①R、C组IL-3Rα阳性率及功能明显降低, 伤后14d无明显恢复。②R、C组IL-3Rα的平均含量明显降低, 伤后14d开始回升, 且与BMNC计数间存在明显正相关。③B组IL-3Rα阳性率、平均含量及功能无显着改变。结论 ①IL-3Rα表达量在放烧复合伤小鼠造血损伤及造血恢复中起一定作用。②IL-3R的功能在放烧复合伤小鼠造血损伤中起一定作用, 但其恢复晚于表达量的恢复。③15%Ⅲ度烧伤对IL-3R的作用不明显；放烧复合伤基本上表现出放射损伤的特点。 |
| 英文摘要: |
| Objective To study the changes of expression and function of IL-3R of BMNC and explore the role of IL-3R in the hematopoietic regulation in combined irradiation burn injury in mice. Methods Mice were randomized into normal (N),irradiation (R),burn (B) and combined irradiation burn injury (C) groups. Changes of positive rate,mean contents and function of IL-3R α subunit were determined on the 1st,3rd,7th and 14th days post injury. Relationships between BMNC counts and positive rate,mean contents, function of IL-3R were also evaluated. Results ① Compared with Group N the positive rate and function of IL-3R α subunit of BMNC were significantly decreased in groups R,C,and maintained low during the first 14 days. ② The mean contents of IL-3R α subunit of BMNC were significantly lower in groups R,C than that in group N at first,and then began to increase on the 14th day but did not reach the normal level. There was a significant positive correlation between mean contents of IL-3R α subunit and BMNC counts in all groups. ③ No significant changes in positive rate,mean contents and function of IL- 3R were found in group B. Conclusions ① Expression of IL-3R α of BMNC plays an important role in the damage and recovery of hematopoiesis in combined irradiation burn injury in mice. The funciton of IL-3R of BMNC is also important in the damage of hematopoiesis,but its functional recovery occurs later than that of its content in combined irradiation burn injury in mice. ② There are no significant changes in IL-3R of BMNC after Ⅲ degree burn with 15% TBSA,so combined irradiation burn injury might exert a similar effect on IL-3R as irradiation alone does. |
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