| 朱寿,彭肖东.147Pm照射对人白血病HL-60细胞bc-l2和bax基因表达的研究[J].中华放射医学与防护杂志,2000,20(1):33-35 |
| 147Pm照射对人白血病HL-60细胞bc-l2和bax基因表达的研究 |
| Effect of 147Pm β-irradiation on bcl-2 gene and bax gene expression in human leukemia cells |
| 投稿时间：1999-04-01 |
| DOI： |
| 中文关键词: 147Pm HL-60细胞 基因表达 bc-l2/bax基因 免疫组织化学 RNA点杂交 |
| 英文关键词:Pm HL-60 cells Gene expression bcl-2 gene bax gene Immunohistochemistry RNA dot blot hybridization |
| 基金项目:国家自然科学基金资助项目(39470389) |
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| 中文摘要: |
| 目的 研究了147Pm照射对人白血病HL60细胞bcl-2和bax基因表达作用。方法 运用免疫组织化学技术探讨147Pm对HL-60细胞bcl-2/bax的蛋白表达, 以及RNA分子杂交技术探讨147Pm对HL-60细胞bcl-2mRNA的转录水平表达。结果 发现对照HL-60细胞中bcl-2蛋白呈高度表达, 为88%;在147Pm照射下, 可下调至53%.bax在对照细胞中的表达很低；147Pm作用后, 未见明显改变。而受147Pm照射后, 可使HL-60细胞中bcl2mRNA表达呈明显下调, 并随受照射时间的延长, 下调作用更加显着。结论 147Pm诱发人白血病HL-60细胞的凋亡作用, 与其下调凋亡相关基因bcl-2的表达相关联。 |
| 英文摘要: |
| Objective To study the effect of exposure to a pure β radiator 147 Pm on bcl-2 gene expression in human leukemia HL-60 cells. Methods Immunohistochemical technique was used to identify the expression of bcl-2 and bax proteins in HL-60 cells,as well as RNA dot blot hybridization to show the expression of bcl-2 mRNA in HL-60 cells. Results The expression of bcl-2 protein was as high as 88% in control human HL-60 cells. Down regulation of bcl-2 protein expression which lowered to 53% was noted after exposure to147 Pm. The expression of bax protein was low in control cells, and after irradiation it did not obviously change. An obvious down regulation of bcl-2 mRNA expression was found in irradiated HL-60 cells. With increasing time of 147 Pm exposure, the down regulation of bcl-2 became more evident. Conclusion The apoptotic action of 147 Pm β-rays in human HL 60 cells is associated with the down regulation of the apoptosis suppressing gene bcl-2. |
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