| 叶飞,刘树铮.不同剂量X射线对同步化HeLaS3细胞周期的影响[J].中华放射医学与防护杂志,1999,19(6):381-384 |
| 不同剂量X射线对同步化HeLaS3细胞周期的影响 |
| Changes in cell cycle progression of HeLaS3cells synchronized after X-ray irradiation |
| 投稿时间：1998-09-08 修订日期：1999-04-08 |
| DOI： |
| 中文关键词: X射线 细胞周期 同步化 HeLaS3细胞 G2阻滞 |
| 英文关键词:X-rays Cell cycle Synchronization HeLaS3 cells G2 delay |
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| 摘要点击次数: 2735 |
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| 中文摘要: |
| 目的 研究不同剂量X射线对同步化HELaS3细胞周期的影响,为进一步探讨其分子调控机理和临床肿瘤放疗提供基础资料。方法 采用胸苷(TdR)双阻断法和流式细胞术(FCM)检测了HELaS3细胞同步化后分别于其细胞周期各时相进行75mGy和2.0GyX射线照射以及于G2+M期进行0.025～2.0Gy照射后其细胞周期进程的变化。结果 2.0Gy照射时细胞无论处于G0/G1、S期还是G2+M期,从释放点后9～15小时内均发生明显的S期延迟和G2阻滞,但其中以G2期照射者阻滞最显着；于G0/G1和G2+M期进行75mGy照射,分别在9和12h时发生一过性的明显的G2阻滞,至11和15h时完全从这一阻滞中脱离,甚至促进了其细胞周期的进程；而在S期进行75mGy照射时,并没有发生这种阻滞,反而加速了其细胞周期由G2+M→G0/G1的移行,其中尤以9h时最为显着。剂量效应关系研究表明,于G2+M期进行0.025～2.0Gy照射后,在释放点后11h时均发生G0/G1期细胞数减少,G2阻滞,且有剂量依赖性,而S期细胞数的变化在0.025～0.1Gy和0.5～2.0Gy照射时截然相反。结论 于G0/G1、G2 + M期进行照射，即使剂量低至25mGy也可发生G2阻滞，但于S期进行照射时，在低剂量范围内(25～100mGy)没有这种现象发生。 |
| 英文摘要: |
| Objective To observe the effect of different doses of X-rays on cell cycle progression of synchronized HeLaS3 cells. Methods Using a double block method with thymidine and flow cytometric analysis,the changes in cell cycle progression of synchronized HeLaS3 cells were examined after 75 mGy and 2.0 Gy X irradiation in G0/G1,S and G2+M phases respectively,and the dose response relationship given in G2/M phase was analyzed after irradiation. Results The S and G2 phases occurred late 9 15h after the releasing point regardless of whether HeLaS3 cells were irradiated with 2 Gy in G0/G1,S or G2+M phases.HeLaS3 cells underwent a G2 arrest 9 and 12h after the releasing point when 75 mGy irradiation was administered in G0/G1 and G2+M phases,and this delay skipped completely at 12 and 15h,respectively.Moreover,the cell cycle progression was accelerated. However,there was no G2 delay at 9 and 11h when the cells were irradiated with 75 mGy in S phase,and in this case the cell cycle progression from G2/M to G0/G1 phase was accelerated.A study on the dose effect relationship showed that the number of G0/G1 phase cells decreased,and G2 delayed after 0.025-2 0.Gy irradiation given in G2/M phase,and the delay was dose dependent.However,the changes in the number of S phase cells were different between low (0.025-0.1 Gy) and higher(0.5-2.0 Gy) doses. Conclusion G2 delay is a very radiosensitive parameter,which occurs in HeLaS3 cells after X ray irradiation with a dose as low as 25 mGy when administered in G0/G1 and G2+M phases,but this delay only occurs with doses above 0.1 Gy when the cells are irradiated in S phase. |
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