中华放射医学与防护杂志

白蕴红,王德文,崔雪梅,等.川芎嗪和硫氮唑酮对照射后NIH/3 T3成纤维细胞增殖及bFGF表达的影响[J].中华放射医学与防护杂志,1999,19(6):379-380.BAI Yunhong,WANG Dewen,CUI Xuemei,et al.Effect of ligustrazine and diltiazem on fibroblast proliferation and expression of basic fibroblast growth factor following ⁶⁰Co γ ray irradiation[J].Chin J Radiol Med Prot,1999,19(6):379-380

川芎嗪和硫氮唑酮对照射后NIH/3 T3成纤维细胞增殖及bFGF表达的影响

Effect of ligustrazine and diltiazem on fibroblast proliferation and expression of basic fibroblast growth factor following ⁶⁰Co γ ray irradiation

投稿时间：1998-05-07 修订日期：1999-04-06

DOI：

中文关键词: 照射成纤维细胞碱性成纤维细胞生长因子

英文关键词:Irradiation Fibroblast Fibroblast growth factor

基金项目:

作者	单位
白蕴红	100850 北京放射医学研究所
王德文	100850 北京放射医学研究所
崔雪梅	100850 北京放射医学研究所
宋良文	100850 北京放射医学研究所
夏国伟	100850 北京放射医学研究所
刘玉福	北京同仁医院眼科

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中文摘要:

目的探讨川芎嗪及硫氮唑酮对照射后NIH/3T3成纤维细胞增殖及bFGF表达的影响,为防治放射性肺纤维化的药物研究提供实验依据。方法采用MTT法测定离体培养的NIH/3T3成纤维细胞增殖程度并用免疫细胞化学ABC方法观察bFGF蛋白表达改变。结果不同组MTT法测得光密度值,对照组为0.71±0.22;照射组(Ⅰ)为1.03±0.28;川芎嗪+照射组为0.47±0.15;照射组(Ⅱ)为1.05±0.21;硫氮唑酮+照射组为2.02±0.21;不同组bFGF蛋白表达平均光密度：对照组为0.028±0.005;照射组为0.044±0.006;川芎嗪+照射组为0.029±0.001;硫氮唑酮+照射组为0.036±0.003.结论川芎嗪明显抑制γ射线照射后NIH/3T3成纤维细胞的增殖及bFGF蛋白表达,硫氮唑酮则不具有这一作用。

英文摘要:

Objective In order to provide experimental basis for drug research of preventing and treating radiation pulmonary fibrosis,the authors observed the effect of ligustrazine and diltiazem on fibroblast proliferation and expression of basic fibroblast growth factor (bFGF) following ⁶⁰ Co γ ray irradiation. Methods NIH/3T3 fibroblast proliferation and expression of bFGF were observed respectively by using MTT[3-(4,5-dimethylthiazo(-2-zyl) 2,5-diphenyl tetrazolium bromide)] method and ABC immunocytochemistry staining. Results The optical densities of bFGF were 0.71+0.22 for control group, 1.03+0.21 for irradiation group (Ⅰ), 0.47+0.15 for ligustrazine+irradiation group,1.05+0.21 for irradiation group (Ⅱ),and 2.02+0.21 for diltiazem+irradiation group.The mean optical densities of bFGF were 0.028+0.005,0.044+0.006,0.029+0.001,and 0.0036+0.003 for control group,irradiation group,ligustrazine+irradiation group,and diltiazem+irradiation group, respectvely. Conclusion Ligustrazine can inhibit the NIH/3T3 fibroblast proliferation and expression of bFGF,but diltiazem does not show such an effect.

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