| 史剑慧,程文英,邵松生,冯嘉林,张有望.鼻咽癌的放射敏感性预测初探[J].中华放射医学与防护杂志,1999,19(5):347-349 |
| 鼻咽癌的放射敏感性预测初探 |
| Combined detection of radiosensitivity of nasopharygeal carcinoma |
| 投稿时间：1999-01-12 修订日期：1999-05-10 |
| DOI： |
| 中文关键词: 鼻咽癌 放射敏感性 |
| 英文关键词:Nasopharygeal carcinoma Radiosensitivity |
| 基金项目:国家八五攻关分题基金 |
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| 摘要点击次数: 2749 |
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| 中文摘要: |
| 目的 探索原位缺口平移、早熟凝集染色体分析和胞质分裂阻断微核法等技术成为判断鼻咽癌放射敏感性预测指标的可能性。方法 以原位缺口平移法研究了鼻咽癌细胞株CNE、CNE-2Z细胞对γ射线的放射效应；以早熟凝集染色体分析技术和胞质分裂阻断微核法研究了鼻咽癌活检组织的放射敏感性。结果 在0～8Gy之间,CNE及CNE-2Z的脱氧三磷酸核苷(dNTP)掺入率与照射剂量间有良好的剂量效应关系,来源于低分化肿瘤的CN-2Z细胞株的放射敏感性高于CNE;32例鼻咽活检标本进行早熟凝集染色体分析,其早熟凝集染色体周期分布与鼻咽癌患者放疗疗效有明显关系,临床上对放疗呈高敏的患者,晚G1细胞比例显着升高；胞质分裂阻断微核法提示20例患者照射后微核形成有显着个体差异,其中低反应者2例有复发倾向。结论 实验结果提示上述3项指标可望成为综合判断鼻咽癌放射敏感性的重要参数。 |
| 英文摘要: |
| Objective Comparisons of radiosensitivity in cells derived from different individual or pathologic types of nasopharygeal carcinoma were carried out to evaluate the combined detection of radiosensitivity of nasopharygeal carcinoma. Methods The radiosensitivity of nasophargeal carcinoma cell lines CNE and CNE-2Z which had been treated with γ-rays were detected by in situ nick translation(ISNT).The premature chromosome condensation(PCC) technique and cytokinesis-block micronucleus(CBMN) method were used to study radiation-induced cytogenetic damage in cell derived from biopsy samples of 52 patients with nasopharygeal carcinoma. Results The radiosensitivity was lower for CNE which was highly differentiated cancerous cell line than that of CNE-2Z whose differentiation degree was poorer.When the cell lines were exposed to from 1 to 8 Gy of γ-rays, the incorporation rate of dNTP of the two cell lines increased linearly with increasing dosage.The sutdy of PCC assay in biopsy samples from 32 patients with nasopharygeal carcinoma suggested that when the cells were in late G-1 phase and had higher survival rate after irradiation, they were more sensitive than those in other phases.The results of CBMN study in 20 patients also exhibited that the lower micronucleus rate, the easier recurrence of cancer. Conclusion The combined detection of radiosensitivity in cells of nasopharygeal carcinoma may provide potential tool for predicting radiosensitivity of carcerous tissues treated with γ-rays. |
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