| 徐永忠,赵经涌,劳勤华.晚期混合裂变产物内照射诱发大鼠外周血淋巴细胞HPRT基因位点突变与微核的比较研究[J].中华放射医学与防护杂志,1999,19(5):337-340 |
| 晚期混合裂变产物内照射诱发大鼠外周血淋巴细胞HPRT基因位点突变与微核的比较研究 |
| Mutations at HPRT loci and micronuclei induced by late fission products in peripheral blood lymphocytes of rats |
| 投稿时间：1998-08-12 修订日期：1998-11-17 |
| DOI： |
| 中文关键词: 混合裂变产物 HPRT基因突变 微核 剂量效应 剂量率效应 |
| 英文关键词:Late fission products HPRT gene mutation Micronucleus Dose effects Doserate effects |
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| 中文摘要: |
| 目的 探索HPRT基因位点突变检测作为辐射生物剂量计的可行性。方法 运用多核细胞法及胞质分裂阻断微核(CBMN)法研究大鼠外周血淋巴细胞的HPRT基因位点突变及微核与累积剂量、剂量率之间关系。结果 静注晚期混合裂变产物后第1天(累积剂量1.73cSv),与对照组相比,HPRT位点变异频率(Vf)、微核细胞(MNC)率、微核(MN)率均已显着增加(P<0.01).HPRTVf、MNC率、MN率与累积剂量间均可拟合成对数回归方程：Y=a+blnX.在总累积剂量近似相等条件下,HPRTVf、MN率与剂量率之间的关系均可用函数Y=a+blnX表示。HPRTVf与MN率间存在线性相关。结论 HPRT基因位点对晚期混合裂变产物内照射非常敏感,有明显的剂量效应关系。HPRT基因突变检测可望成为评估电离辐射损伤的生物剂量计。 |
| 英文摘要: |
| Objective The probability of mutations at HPRT loci used as a biological dosimeter was studied. Methods With the technique of multinuclear cell and cytokinesis-block micronucleus (CBMN) method, the relationships between mutations at HPRT loci, micronucleus and accmulative doses and dose-rates were studied. Results On the first day after intravenous injection of late fission products(accumulative dose 1.73cSv), by comparison with the control group, the HPRT variant frequencies, micronucleate cell frequencies and micronucleus frequencies increased significantly( P <0.01).There existed relationships between variant frequencies at HPRT locus, micronuclear cell frequencies, micronucleus frequencies and accumulative doses, which could be described by the following regression equation:Y=a+blnX, respectively.At the same accumulative dose, both the relationships between HPRT variant frequencies, micronucleus frequencies and dose-rates could be expressed by the logarithmic regression equation:Y=a+blnX.A good linear relationship between HPRT variant frequencies and micronucleus frequencies was observed. Conclusion HPRT gene locus is an endpoint very sensitive to injurious effects resulting from late fission products and has a good dose-response relationship. |
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