崔玉芳,杨红,高亚兵,熊呈琦,夏国伟,王德文.白介素-1对正常和受照射的NIH/3T3成纤维细胞增殖的影响[J].中华放射医学与防护杂志,1999,19(5):334-336 |
白介素-1对正常和受照射的NIH/3T3成纤维细胞增殖的影响 |
Effects of interleukin|1 on proliferation of normal and irradiated NIH/3T3 fibroblasts |
投稿时间:1998-07-26 修订日期:1998-09-14 |
DOI: |
中文关键词: IL-1 成纤维细胞 MTT AgNOR_S Ⅲ型胶原 DNA合成 |
英文关键词:Interleukin1 Fibroblasts Gamma radiation Type Ⅲ collagen DNA synthesis |
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中文摘要: |
目的 观察IL-1对成纤维细胞生长的影响及其作用机理,为从细胞因子角度开展抗纤维化研究提供依据。方法 用MTT比色、AgNORS银染和免疫组化技术,观察正常和受照NIH/3T3成纤维细胞(60Coγ源一次照射,吸收剂量30Gy)生长增殖活性、AgNORS和DNA合成以及Ⅲ型胶原表达。结果 IL-1浓度为100×103U/L,培养第3天时,成纤维细胞数量和增殖活性(A值)为对照值的1.4和1.3倍(照射组A值为对照的1.1倍);AgNORS颗粒数和S期细胞比例分别升高至对照值的2.9和2.0倍;而Ⅲ型胶原表达阳性细胞数为对照值的1.9倍。结论 在一定浓度范围内,IL-1能促进成纤维细胞的增殖活性,DNA合成和Ⅲ型胶原表达。 |
英文摘要: |
Objective Effects of interleukin-1(IL-1) on proliferation of fibroblasts and their mechanism were analysed in order to provide experimental basis for studying antifibrotic activity from the point of cytokines. Methods MTT colorimetry, a silver colloid techniqiue of AgNOR-S, and immuno-histochemical methods of anti-type Ⅲ collagen and anti-bromodeoxyuridine(BrdUrd) were used to observe the proliferative activity, AgNOR-S and DNA synthesis and expession of type Ⅲ collagen in normal and irradiatd (30 Gy single irradiation by60Co γ-rays) fibroblasts. Results When IL-1 of 100×10+3 U/L was used, the counts and optical density(A) value of normal fibroblasts were 1.4 and 1.3 times higher than those of control group respectively, at the third day after culturing.At the same time, the mean number of AgNOR-S and percentage of normal fibroblasts in S-phase were 2.9 and 2.0 times higher than those of control group, while the number of type Ⅲ collagen positive fibroblats increased by 1.9 times. Conclusion In a definite range of concentration, IL-1 could promote proliferative activity, AgNOR-S and DNA synthesis and expression of type Ⅲ collagen of fibroblasts. |
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