| 李雨,鲁颖,郑秀龙,罗成基,蔡建明,孟祥顺,赵芳,高建国,赵岚.Wort mannin对细胞的辐射增敏作用[J].中华放射医学与防护杂志,1999,19(5):330-333 |
| Wort mannin对细胞的辐射增敏作用 |
| Cellular radiosensitization by wortmannin |
| 投稿时间：1998-12-14 修订日期：1999-03-24 |
| DOI： |
| 中文关键词: Wortmannin 放射增敏 |
| 英文关键词:Wortmannin Radiosensitization |
| 基金项目: |
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| 中文摘要: |
| 目的 研究磷脂酰肌醇3激酶的特异性抑制剂Wortmannin(WT)对细胞的放射增敏效应,以探索放射增敏信号传导途径。方法 采用体外细胞克隆培养法观察不同浓度WT在不同时间对3种细胞的放射增敏效应。结果 50μmol/L以上浓度的WT在照射后6小时内对LP3细胞呈较明显的增敏效应,剂量存活曲线D0增敏比在2左右；50μmol/LWT对HeLa细胞和SP/20细胞均呈明确增敏效应。结论 WT是一种新型放射增敏剂。 |
| 英文摘要: |
| Objective To examine the radiosensitization possibility of wortmannin(WT) on some cell lines. Methods Cells were prepared with WT or DMSO(solvent) for 1h, and irradiated at the indicated dose.At different times after irradiation, the cells were washed to remove the WT and allowed to grow for 7 days, and colonies of more than 50 cells were examined under low power microscope. Results Marked radiosensitizing effect of WT in concentration over 50 μmol/L on LP cells within 6 h after radiation exposure was displayed, the D0 radiosensitization ratio in the dose survival curve being around 2.WT in concentration 50 μmol/L had definite radiosensitizing effect on HeLa and SP/20 cells. Conclusion WT is a noveldiosensitizer. |
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