| 梁克,何少琴,冯炎,唐锦华,冯勤富,沈瑜,殷蔚伯,徐国镇,刘新帆,王绿化,高黎.快中子诱导人鼻咽癌细胞系凋亡的研究[J].中华放射医学与防护杂志,1999,19(5):319-321 |
| 快中子诱导人鼻咽癌细胞系凋亡的研究 |
| Apoptosis of nasopharyngeal carcinoma cell line(CNE-2) induced by neutron irradiation |
| 投稿时间：1999-03-21 修订日期：1999-06-13 |
| DOI： |
| 中文关键词: 中子射线 凋亡 鼻咽癌 |
| 英文关键词:Neutron beams Apoptosis Nasopharyngeal carcinoma |
| 基金项目:国家教委博士点课题;国家自然科学基金课题;国家科委“九五”攀登计划 |
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| 中文摘要: |
| 目的 研究中子不同剂量照射人鼻咽癌(CNE-2)细胞凋亡发生特点及与X射线所致凋亡的差异。探讨凋亡在中子治疗肿瘤中的作用及临床意义。方法 采用琼脂糖凝胶电泳及DNA特异性荧光染色方法(Hoechst33342)检测照射后不同时间人鼻咽癌(CNE-2)细胞。结果 发现中子可诱导人鼻咽癌细胞发生凋亡,这种凋亡发生存在着一定的时间剂量相关性。在相同剂量照射下,同一时间点上,中子照射所致的凋亡反应强于X射线所致的凋亡。结论 快中子照射离体细胞可引起较强的凋亡反应。中子杀伤肿瘤的机理可能也是主要通过凋亡途径来实现的。 |
| 英文摘要: |
| Objective To study the apoptotic response of the nasopharyngeal carcinoma cell line (CNE-2) induced by neutron irradiation. Methods CNE-2 cells were cultured as usual.Using the techniques of DNA agarose gel electrophoresis and DNA special fluorescent staining,the status of apoptosis in CNE-2 cells after neutron irradiation was detected. Results It was shown that the apoptosis can be induced in CNE-2 cell after neutron radiation.Six hrs, after different doses of neutron(0/0.667/1.333/2.000/ 2.667/3.333Gy) and X ray (0/2/4/6/8/10 Gy) irradiation the apoptotic rates were 2.4%, 6.3%, 7.1%, 9.5%, 13.5%, 14.6% and 2.4%, 3.8%, 5.7%, 7.8%, 10.4%, 11.7%, respectivelyat 48hrs they were 18.3%, 21.5%, 22.8%, 29.3%, 34.2% and 13.7%, 17.6%, 21.3%, 25.6%, 28.9%, respectively.At 10hrs after neutron irradiation the DNA ladder of apoptosis could be detected between 0.667- 3.333 Gy doses in CNE-2 cells by DNA agarose gel electrophoresis. Conclusion Neutron radiation can induceapoptosis in tumor cells.Compared with the X ray, neutron induces apoptosis in larger extent than X ray in the same conditionmeanwhile, apoptosis after irradiation is dose and time dependent. |
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