| 杨婷,饶志仁,吴声伶,吴鸿勋,鞠躬.γ-刀不同剂量一次单侧照射大鼠尾壳核后前脑星形胶质细胞的变化[J].中华放射医学与防护杂志,1999,19(5):316-318 |
| γ-刀不同剂量一次单侧照射大鼠尾壳核后前脑星形胶质细胞的变化 |
| Changes of astrocytes in rat forebrain after unilateral irradiation of nucleus caudate putamen with different single doses of gamma knife |
| 投稿时间：1998-11-05 修订日期：1999-03-11 |
| DOI： |
| 中文关键词: γ刀 尾壳核 GFAP 星形胶质细胞 免疫组织化学 |
| 英文关键词:Gamma knife Caudate putamen nucleus Glial fibrillary acidic protein Astrocytes Immunohistochemistry |
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| 中文摘要: |
| 目的 作者研究γ-刀不同剂量定位照射大鼠脑尾壳核中部后前脑内星形细胞(AS)形态和数量的变化以探讨γ-刀的放射生物效应,为临床防护提供依据。方法 应用免疫组织化学ABC法以AS的中型丝主要构成成分---胶质细胞原纤维酸性蛋白(GFAP)作为免疫染色标志观察照射后90天GFAP的表达及变化情况。结果 ①吸收剂量10～30Gy时,AS在靶区内大量增生,细胞肥大和变性,与对侧形成明显对比；40～60Gy时照射靶区AS肥大和变性增多；70～100Gy时,靶区出现边界明确的坏死区,离坏死区越近,细胞变性越明显；靶区和隔区出现明显的水肿,细胞胞体肥大明显。②从低剂量到高剂量,血管扩张变形渐趋严重,80Gy时,血管内膜出现凹凸不平。③在远离照射部位的脑区,双侧反应差别较小。结论 低剂量伽玛刀局部照射,90天时可通过特异性抗体检测出AS的数量和形态改变；靶区的损伤程度和剂量成正相关。 |
| 英文摘要: |
| Objective To investigate variations in morphology and number of astrocytes in the rat forebrain after the caudate putamen nucleus was irradiated by differant single doses of gamma knife from 10Gy to 100Gy. Methods Immunohistochemical ABC method was used to stain glia fibrillary acidic protein(GFAP). Results Firstly, at dosage of 10-30Gy, the number of astrocytes was increased remarkably in the target area and the non target areas displayed much less reactive cellsand when irradiated with 40-60Gy, the brain showed more hypertrophic and degenerated astrocytes in the target areawhereas at dosage of 70-100Gy, necrotic hole began to appear and became bigger with an increasing dosage.The nucleus caudate putamen and septal area became swollen and blood vessels dilated, which tended to increase with increasing dosage.Lastly, in areas not directly irradiated by gamma knife, the ipsilateral and contralateral sides showed a less difference in GFAP expression in the forebrain. Conclusion Degree of damage in target area is positively relative to the dosage. |
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