| 胡迎春,张开泰,吴德昌,等.PCR快速筛选质粒cDNA文库方法的建立[J].中华放射医学与防护杂志,1999,19(5):310-312.HU Yingchun,ZHANG Kaitai,WU Dechang,et al.A rapid method for screening arrayed plasmid cDNA library by PCR[J].Chin J Radiol Med Prot,1999,19(5):310-312 |
| PCR快速筛选质粒cDNA文库方法的建立 |
| A rapid method for screening arrayed plasmid cDNA library by PCR |
| 投稿时间：1998-10-08 修订日期：1999-02-25 |
| DOI： |
| 中文关键词: PCR 文库筛选 |
| 英文关键词:Polymerase chain reaction cDNA library screening |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的 建立一种快速、有效地筛选质粒cDNA文库的方法-PCR法。方法 将质粒cDNA文库重组子进行矩阵排列,然后用特异引物进行PCR逐级筛选以获得目的克隆。结果 经两轮筛选(约1周的时间)获得4株阳性克隆。结论 PCR矩阵筛库法不仅适用于常规cDNA克隆的筛选,还适用于筛选较短的cDNA片段,比常规的筛库方法更为灵敏、特异、高效。 |
| 英文摘要: |
| Objective To develop a PCR-based method for rapid and effective screening of arrayed plasmid cDNA library. Methods The plasmid cDNA library was arrayed and screened by PCR with a particular set of primers. Results Four positive clones were obtained through about one week. Conclusion This method can be applied to screening not only normal cDNA clones, but also cDNA clones-containing small size fragments.This method offers significant advantages over traditional screening method in terms of sensitivity, specificity and efficiency. |
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