| 朱寿彭,肖东,韩晓枫.DNA凝胶电泳和放射自显影研究153Sm诱发骨肉瘤细胞凋亡[J].中华放射医学与防护杂志,1999,19(5):307-309 |
| DNA凝胶电泳和放射自显影研究153Sm诱发骨肉瘤细胞凋亡 |
| DNA gel electrophoretic and microautoradiographic studies on apoptosis in bone tumor cells after exposure to 153 Sm|EDTMP |
| 投稿时间：1998-05-15 修订日期：1998-10-20 |
| DOI： |
| 中文关键词: DNA凝胶电泳 微观放射自显影 153SmEDTMP 骨肉瘤细胞 凋亡 |
| 英文关键词:DNA gel electrophoresis Microautoradiography 153 Sm-EDTMP Internal irradiation Bone tumor cells Apoptosis |
| 基金项目:国际原子能机构资助 |
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| 中文摘要: |
| 目的 研究受153Sm-EDTMP内照射治疗时诱发骨肉瘤细胞凋亡的DNA损伤特征,以及153Sm核素在瘤细胞中的行径特性。方法 观察了153Sm-EDTMP内照射不同时间,诱发骨肉瘤细胞凋亡的DNA凝胶电泳和微观放射自显影的示踪动态。结果 研究发现,在153Sm-EDTMP内照射作用下,骨肉瘤细胞的DNA呈现出在核小体间断裂的阶梯状条带形成的凋亡特征,放射性核素153Sm可迅速透过瘤细胞膜,也可被瘤细胞吞噬,呈亲膜性积聚和膜包裹着的凋亡小体呈现。结论 153Sm-EDTMP内照射治疗时,可诱发骨肉瘤细胞凋亡发生,其诱发凋亡的程度随153Sm内照射作用时间延长而增升。 |
| 英文摘要: |
| Objective To study the characteristics of injury in apoptotic bone tumor cells and behavior of radionuclide 153Sm in tumor cells. Methods DNA gel electrophoresis and microautoradiographic tracing of apoptotic bone tumor cells at different intervals after 153Sm-EDTMP internal irradiation. Results Bone tumor cells internally irradiated with 153 Sm-EDTMP displayed characteristics of DNA ladder formation in apoptotic cells. 153 Sm could permeate through tumor cell membrane and be phagocytized by the tumor cells, showing membrane-seeking and membrane bounded apoptotic bodies condensation. Conclusion Progression of apoptosis in bone tumor cells induced by 153 Sm-EDTMP is dependent on the time elapse of 153 Sm internal irradiation. |
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