| 项莺松,杨如俊,蔡建明,等.骨髓细胞经酸性成纤维细胞生长因子孵育后程序移植的实验研究[J].中华放射医学与防护杂志,1999,19(5):303-306.XIANG Yingsong,YANG Rujun,CAI Jianming,et al.Scheduled transplantation of bone marrow cells preincubated with acidic fibroblast growth factor(aFGF)[J].Chin J Radiol Med Prot,1999,19(5):303-306 |
| 骨髓细胞经酸性成纤维细胞生长因子孵育后程序移植的实验研究 |
| Scheduled transplantation of bone marrow cells preincubated with acidic fibroblast growth factor(aFGF) |
| 投稿时间：1998-07-31 修订日期：1998-10-14 |
| DOI： |
| 中文关键词: 骨髓移植 酸性成纤维细胞生长因子 移植物抗宿主病 |
| 英文关键词:Bone marrow transplantation Graft versus host disease Acidic fibroblast growth factor |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的 充分利用酸性成纤维细胞生长因子(aFGF)促进造血重建的作用,以进一步完善程序移植新方法。方法 以昆明种小鼠急性放射病为模型,进行了骨髓细胞(BMC)经自牛脑中制备的aFGF孵育后程序移植对造血重建、急性移植物抗宿生病(GVHD)的研究。结果 aFGF孵育后程序移植BMC4×106,存活率达40%,比单纯一次移植BMC1×107组高(30%),但不如单纯骨髓程序移植BMC4×106组(60%);另一方面,外周血白细胞、骨髓有核细胞计数、CFU-E、CFU-GM、CFU-S比一次骨髓移植1×107组和单纯骨髓程序移植BMC4×106回升快,而CFU-F回升无差别；GVHD较一次骨髓移植1×107组轻,较骨髓程序移植4×106重。结论 引起GVHD的主要原因是异基因T淋巴细胞,通过aFGF孵育后程序移植可以更充分地发挥其促进造血重建的作用,而减轻GVHD. |
| 英文摘要: |
| Objective To develop a new method of bone marrow scheduled transplantation (BMST) by making use of the effects of acidic fibroblast growth factor (aFGF) on improving hematopoiesis. Methods The scheduled transplantation of bone marrow cells preincubated with aFGF (aFGF BMST) was carried out to study the effects of aFGF on hematopoietic reconstitution and reducing acute graft versus host disease (GVHD) in acute radiation disease model of Kungming mice. Results The survival rate of the group of aFGF-BMST mice with 4×106BMXs was 40%, which was higher than the survival of the group of BMT with 1×107BMCs alone (30%), but was lower than the survival of the group of BMST with 4×106 BMCs. On the other hand, the recovery rates in numbers of leucocytes, nucleated cells and CFU-E, CFU-GM, CFU-S were faster than those in the group of BMT with 1×107 BMCs alone and in the group of BMST with 4×106 BMCs.In addition, the severity of GVHD in the group of aFGF-BMST mice with 4×106 BMCs was lower than that in the group of BMT with 1×107 BMCs alone but was higher than that in the group of BMST with 4×106 BMCs. Conclusion Although aFGF can activate heterogneous T cells to cause GVHD, there is prospect of making full use of the effects of aFGF on improving hematopoisis and reducing the side effects of aFGF leading to GVHD through scheduled transplantation of bone marrow cells preincubated with aFGF. |
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