| 李小明,童新,宋天保,孙志贤.Caspase-3基因在凋亡HL-60细胞及肿瘤细胞中的表达[J].中华放射医学与防护杂志,1999,19(5):297-299 |
| Caspase-3基因在凋亡HL-60细胞及肿瘤细胞中的表达 |
| Expression of caspase 3 gene in apoptotic HL 60 cell and different human tumor cell lines |
| 投稿时间：1998-09-17 修订日期：1999-01-22 |
| DOI： |
| 中文关键词: γ射线 细胞凋亡 caspase3 基因表达 |
| 英文关键词:radiation Apoptosis Caspase-3 Gene expression |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的 明确caspase3基因在凋亡前后HL-60细胞及不同肿瘤细胞系中的表达特性。方法 用Northern杂交技术检测γ射线诱发的凋亡前后HL-60细胞和6种肿瘤细胞中caspase-3的mRNA.结果 caspase-3基因在白血病细胞株HL-60、CEM和K562细胞及视母细胞瘤细胞SHSY5Y中表达高于宫颈癌细胞HeLa及乳腺癌细胞MCF7;在凋亡HL-60细胞表达高于未照射对照HL-60细胞。结论 caspase-3表达水平与细胞寿命、对辐射敏感性及细胞凋亡的发生有关。 |
| 英文摘要: |
| Objective To research the expression of caspase-3 gene in the apoptotic and the control HL-60 cells and in the different human tumor cell lines. Methods Caspase-3 mRNA in the control and γ radiation induced apoptotic HL-60 cells, and in the 6 types of human tumor cell lines, was analysed by Northern blot. Results The caspase-3 gene transcript was more highly expressed in leukemia cells HL-60, CEM, K562 and neuroblastoma SH-SY5Y than in cervical adenocarcinoma HeLa and breast carcinoma MCF7, and more highly in the radiation induced apoptotic HL-60 than in the control HL-60 cells. Conclusion The high level of expression of caspase-3 may aid the efforts to understand the tumor cell sensitivity to radiation, apoptosis and its inherent ability to survive. |
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