| 桂红星,程文英,史剑慧,等.放射事故受照者血型糖蛋白A突变分析[J].中华放射医学与防护杂志,1999,19(4):272-274.GUI Hongxing,CHENG Wenying,SHI Jianhui,et al.Assay of glycophorin A locus mutation in radiation accident victims[J].Chin J Radiol Med Prot,1999,19(4):272-274 |
| 放射事故受照者血型糖蛋白A突变分析 |
| Assay of glycophorin A locus mutation in radiation accident victims |
| 投稿时间：1998-11-18 修订日期：1999-01-12 |
| DOI： |
| 中文关键词: 血型糖蛋白A(GPA) 单合子NO 纯合子NN 变异率Vf |
| 英文关键词:Glycophorin A locus Hemizygote NO Homozygote NN Variant frequency |
| 基金项目:国家教委博士点资助 |
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| 中文摘要: |
| 目的 研究血型糖蛋白A(GPA, glycophorin Alocus)突变分析用作新的辐射生物剂量计。方法 结合流式细胞分析和单克隆抗体标记技术,检测红细胞糖蛋白的变异。结果 随访全国10例放射源事故受照者,4人为MN杂合体,生物剂量在1.46～2.9Gy, 1人MN弱阳性。GPANO变异率与剂量线性回归曲线有显着意义,曲线斜率41.53×10-6/Gy, 而NN没有显着意义。结论 GPA变异率剂量效应曲线二次线性拟合比一次线性好。二次线性重建远期辐射剂量高于一次模型,用原爆、切尔诺贝利、戈亚尼亚事故参数回顾剂量多偏高。 |
| 英文摘要: |
| Objective Application of glycophorin A locus mutation assay as a new biodosimeter. Methods Using flow cytometry and immunolabeling to detect erythrocytes with variant glycoproteins on the surface membrane. Results Among 10 victims of radiation accidents 4 persons were determined as having MN heterozygotes with estimated dose of 1.46-2.9 Gy, and one suspected of having MN. Hemizygote NO variant frequency showed significant doseresponse linear regression with slope of 41.53x10-6/Gy, while NN showed no significant regression. Conclusion Doseresponse data are more fitted to the linearquadratic model than to the linear model. Dose reconstructed with the linearquadratic model is higher than that estimated with the latter. And doses estimated with standard curve of Hiroshima and Chernoby1 are higher than those assessed by assay of dicentrics plus rings after the accidents. |
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