| 姚伟强,Luka Milas.Genistein对小鼠小肠上皮细胞照射后再增殖和TGF-α、EGF-R表达的影响[J].中华放射医学与防护杂志,1999,19(4):261-262 |
| Genistein对小鼠小肠上皮细胞照射后再增殖和TGF-α、EGF-R表达的影响 |
| Effects of genistein on repopulation of mouse jejunum crypts and expression of TGF- and EGF-R following irradiation |
| 投稿时间：1998-10-13 修订日期：1999-01-23 |
| DOI： |
| 中文关键词: 小鼠 小肠 照射 Genistein TGF-α EGF-R |
| 英文关键词:Mice Jejunum Irradiation Genistein TGF--α EGF-R |
| 基金项目:美国纪念Mammadi Soubavar特别研究员基金 |
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| 中文摘要: |
| 目的 研究Genistein对小鼠小肠上皮细胞受照射后再增殖和TGF-α、EGF-R表达的影响。方法 实验鼠分两组：一组为对照组,单纯一次接受全身吸收剂量为5Gy的照射；另一组为用药组,照射剂量与方法同对照组,在照射后不同时间注射Genistein.在照射后不同时间内杀死小鼠取出空肠,计数隐窝细胞数和分裂指数。并测定隐窝区和绒毛区TGF -α和EGF R的表达。结果 与对照组比较,用药组小鼠小肠隐窝细胞的分裂指数和细胞数上升幅度较低,用药组TGF- α在隐窝区表达持续升高,而EGF-R在隐窝区的表达则较对照组低。结论 Genistein能明显抑制小鼠小肠上皮细胞受照射后的再增殖,这种影响可能是通过作用于EGF-R的表达起作用。 |
| 英文摘要: |
| Objective To study the effects of genistein on the repopulation of mouse jejunum crypts and expression of TGF- and EGF-R following irradiation. Methods C3Hf/kam mice were treated by radiotherapy plus genistein as the study group or treated by radiotherapy only as the control group.Using jejunum as material, the number of cryptal cells and mitotic index were measured at different time intervals after irradiation.At the same time the expression of TGF- and EGF-R were also tested by immunohistochemical method. Results Comparied with the control group, the number of cryptal cells and mitotic index in the study group were decreased on days 3-4 after irradiation.The expression of TGF- in crypts kept a high level while the expression of EGF-R in crypts decreased after irradiation in the study group. Conclusion The results indicate that genistein can suppress the repopulation of mouse jejunum crypts after irradiation while TGF- and EGF-R play important roles in repopulation of mouse jejunum crypts after irradiation. |
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