| 曹华,葛忠良,张群伟,刘秀珍.rhTPO/GM-CSF融合蛋白对60Co γ射线照射小鼠造血功能的影响[J].中华放射医学与防护杂志,1999,19(4):251-253 |
| rhTPO/GM-CSF融合蛋白对60Co γ射线照射小鼠造血功能的影响 |
| Influence of rhTPO/GMCSF fusion protein on hemopoiesis in mice irradiated with 60 Co rays |
| 投稿时间：1999-02-24 修订日期：1999-05-25 |
| DOI： |
| 中文关键词: 血小板生长因子 粒-巨噬细胞集落刺激因子 融合蛋白 造血活性 |
| 英文关键词:TPO GM-CSF rhTPO/GM-CSF Fusion protein Hematopoietic activity |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的 探索一个新的、具有能同时促进机体红、粒及巨核细胞系造血的细胞因子,以便治疗由化疗和放疗等各种原因引起全血细胞损伤的综合征。方法 所用的细胞因子是本实验室重组的产品,以照射小鼠为模型,给小鼠注射细胞因子后,观察红、粒及巨核细胞的变化。结果 rhTPO/GM- CSF融合蛋白对受照射小鼠血象有恢复作用。结论 融合蛋白具有TPO与GM- CSF双重的生物活性。 |
| 英文摘要: |
| Objective To find a new biological therapy for secondary hematopoietic failure including anemia, infection and hemorrhage after administration of chemotherapeutic drugs etc. Methods hGM-CSF gene was ligated with hTPO gene isolated from human fetal liver mRNA and a new fusion protein rhTPO/GMCSF obtained. Results The new fusion protein could promote recovery of peripheral WBC and PLT of 5.0 Gy irradiated mice.BFU-E, CF-UMeg and CF-UGM in bone marrow of mice after irradiation recovered significantly by treatment with rhTPO/GM-CSF fusion protein for 10 days. Conclusion These results suggest that the new fusion protein has the biological activity of both hTPO and hGM-CSF simultaneously and can stimulate the proliferation of megakaryocyte and granulocyte progenitors. |
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