| 崔玉芳,高亚兵,杨红,熊呈琦,夏国伟,王德文.γ-干扰素和地塞米松对成纤维细胞生长的影响[J].中华放射医学与防护杂志,1999,19(4):244-247 |
| γ-干扰素和地塞米松对成纤维细胞生长的影响 |
| Effects of interferon and dexamathasone on growth of fibroblasts stimulated by interleukin1 |
| 投稿时间：1998-10-27 修订日期：1999-01-09 |
| DOI： |
| 中文关键词: γ-干扰素 地塞米松 IL-1 成纤维细胞 Ⅲ型胶原 增殖抑制 |
| 英文关键词:Interferon-γ Dexamathasone Interleukin-1 Fibroblasts Collagen typeⅢ Proliferation inhibition |
| 基金项目:总后“九五”指令性基金 |
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| 中文摘要: |
| 目的 观察γ -干扰素(IFN -γ)和地塞米松(Dexa)对正常和IL 1-刺激的成纤维细胞生长的影响,旨在从细胞因子角度为抗纤维化有效措施的制定提供依据。方法 用MTT比色、Ag NORs银染和免疫组化技术,观察两种抑制剂对成纤维细胞增殖、AgNORs合成和Ⅲ型胶原表达的影响。结果 250×103U/L浓度的IFN -γ和100μg/L浓度的Dexa均能明显抑制正常和IL -1刺激的成纤维细胞增殖、AgNORs合成和Ⅲ型胶原表达。结论 一定浓度的IFN -γ和Dexa对正常和IL -1刺激的成纤维细胞生长、增殖、AgNORs合成和Ⅲ型胶原表达均显示出明显的抑制作用。 |
| 英文摘要: |
| Objective sOur studies were aimed at providing a basis for effective therapeutic strategy against fibrosis using cytokines through observing the effects of interferon (IFN-r) and dexamathasone (dexa) on growth of normal and interleukin1(IL-1)stimulated fibroblasts. Methods BZ]MTT colorimetry, a silver colloid technique to identify nucleolar organizer regions (AgNORs) and immunohistochemical methods were used to study the effects of the two inhibitors on growth of fibroblasts. Results Growth, proliferation activity, synthesis of AgNORs and expression of collagen type in normal and IL-1stimulated fibroblasts were depressed evidently by IFN-r at 250103 U/L and dexa at 100g/L. Conclusions The authors believe that IFN-r and dexa at certain concentrations can inhibit obviously the growth, proliferation, AgNORs synthesis and collagen type expression of IL-1stimulated fibroblasts. |
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