| 刘炳辰,吴红英,岳井银,穆传杰,周继文.辐射诱发WI-38细胞遗传不稳定性中的mRNA差异显示分析[J].中华放射医学与防护杂志,1999,19(3):180-182 |
| 辐射诱发WI-38细胞遗传不稳定性中的mRNA差异显示分析 |
| A mRNA DD assay in the studies of genetic instability in WI-38 cells induced by radiation. |
| 投稿时间：1998-06-21 修订日期：1998-11-01 |
| DOI： |
| 中文关键词: 遗传不稳定性 mRNA差异显示 |
| 英文关键词:Genetic instability mRNA-DD PCR |
| 基金项目:国家自然科学基金 |
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| 摘要点击次数: 2776 |
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| 中文摘要: |
| 目的 利用mRNA差异显示技术分析在遗传不稳定性产生和传递过程中, 是否有一些特异的基因表达的变化, 并在此基础上了解哪些基因在此过程中起关键作用。方法 人胚胎成纤维细胞WI -38经γ射线05、10、30、50、70Gy照射后8小时及3Gy照射后4、8、24、48、120小时, 提取细胞RNA, 进行mRNA DDPCR, 并将差异表达片段进行再扩增。结果 PCR扩增产物经电泳后, 有14条表达差异的带型出现, 所有差异表达片段均获得了再扩增。结论 结果说明, 某些特异的基因变化参与到了这一过程中, 根据我们以前的实验推测, 很可能是修复基因的改变。目前正在对这些未知片段进行克隆和序列分析。 |
| 英文摘要: |
| Objective To study the changes in expression of some specific genes in the process of formation and of genetic instability and to determine which genes play a critical role in this process. Methods The total RNA from human fetal fibroblast cell WI-38 was extracted 8 hours after irradiation with 0.5、 1.0、 3.0、 5.0、 7.0 Gy γ-rays and at 4, 8, 24, 48, 120 hours after irradiation with 3.0 Gy γ-rays. The mRNA-DD PCR technique was used to analyze gene expression. Results The gel electrophoresis results showed that 14 differential expression bands appeared as compared with the control.All these differential fragments could be reamplified. Conclusion The results show that some specific genes participate in this proces.They might belong to repair genes according to our results observed before.We are going to clone and sequence these unknown fragments in further studies. |
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