| 周晓,吴德昌,程书钧,胡迎春,舒为群,李刚.α粒子诱导人支气管上皮细胞转化相关基因的分离与克隆[J].中华放射医学与防护杂志,1999,19(3):177-179 |
| α粒子诱导人支气管上皮细胞转化相关基因的分离与克隆 |
| Isolation and cloning of genes related to the transformation of immortalized human bronchial epithelial cell line induced by alpha particle radiation |
| 投稿时间：1998-02-24 修订日期：1998-07-14 |
| DOI： |
| 中文关键词: α照射 人支气管上皮细胞 mRNA差异显示 转化相关基因 |
| 英文关键词:α-Radiation Human bronchial epithelial immortalized cell line mRNA differential display Genes related to transformation |
| 基金项目:国家自然科学基金;中国博士后科学基金 |
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| 中文摘要: |
| 目的 探索α粒子照射诱导人支气管上皮细胞转化的相关基因, 并加以克隆。方法 采用了mRNA差异显示技术, 通过辐射诱发转化细胞与同代未经照射细胞的对比, 找出差异cDNA片段。结果 克隆出2gs3, 5gs1, 5gs2, 2gd1, 6gd15个差异片段, 测序, 经文献检索发现除5gs1外均为新序列, 已登录到EMBL库中。结论 发现了细胞转化早期的新序列的4个cDNA片段, 生物学特性与功能有待研究。 |
| 英文摘要: |
| Objective To find out the genes related to transformation of human bronchial epithelial cell line and to clone them. Methods The mRNA differential display method was used. Results Differential cDNA fragments were found.After sequencing and identification in GenBank,4 fragments were found to be novel ones and were registered in EMBL database. Conclusion Four novel cDNA fragments have been found,but their biological characterictics and functions are still to be elucidated. |
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