中华放射医学与防护杂志

苏旭,刘建香,张迎春,等.低剂量辐射诱导小鼠胸腺细胞及其亚组的适应性反应[J].中华放射医学与防护杂志,1999,19(3):174-176.SU Xu,LIU Jianxiang,ZHANG Yingchun,et al.Adaptive response of thymocytes and its subsets induced by low dose whole body irradiation with X-rays in mice[J].Chin J Radiol Med Prot,1999,19(3):174-176

低剂量辐射诱导小鼠胸腺细胞及其亚组的适应性反应

Adaptive response of thymocytes and its subsets induced by low dose whole body irradiation with X-rays in mice

投稿时间：1998-09-14 修订日期：1999-02-01

DOI：

中文关键词: 低剂量辐射适应性反应胸腺细胞亚组

英文关键词:Low dose irradiation Adaptive response Thymocytes subsets

基金项目:国家自然科学基金

作者	单位
苏旭	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室
刘建香	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室
张迎春	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室
鞠桂芝	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室
刘树铮	130021 长春, 白求恩医科大学卫生部放射生物学重点实验室

摘要点击次数: 3463

全文下载次数: 1856

中文摘要:

目的研究低剂量辐射预先照射以及随后大剂量照射后小鼠胸腺细胞T细胞受体(TCR)、CD3、CD4和CD8分子表达的变化。方法 TCR、CD3表达采用间接荧光流式细胞术检测, CD4、CD8表达采用双参数直接免疫荧光流式细胞术检测。结果实验结果表明:单纯15GyX射线全身照射后TCR, CD3阳性细胞数以及CD4^-D8^-, CD4⁺CD8⁺, CD4⁺CD8^-和CD4^-CD8⁺细胞数明显减少, 当1.5GyX射线全身照射前6小时预先照射0.075Gy时, 可明显减轻其后15Gy照射对TCR⁺, CD3⁺, CD4⁺CD8⁺, CD4⁺CD8^-和CD4^-CD8⁺的损伤作用。表现为各亚组细胞数显着高于单纯1.5Gy照射组。CD4^-CD8^-亚组的细胞数无明显变化。结论 0.075GyX射线全身照射能够诱导胸腺细胞TCR⁺, CD3⁺, CD4⁺CD8⁺, CD4⁺CD8^-和CD4^-CD8⁺亚组细胞的适应性反应。

英文摘要:

Objective To study the changes in expression of TCR,CD3 CD4 and CD8 molecules on thymocytes following irradiation with low dose X-rays before a challenge dose in mice. Methods Kunming mice were irradiated with 0.075 Gy (conditioning dose) 6 hours before 1.5 Gy irradiation (challenge dose).The expression of TCR,CD3,CD4 and CD8 were analyzed by flow cytometry 18 hours after 1.5 Gy irradiation. Results It was found that the TCR⁺,CD3⁺,CD4^-CD8⁺,CD4⁺CD8^- and CD4^-CD8 ⁺ positive cells were decreased significantly after only 1.5 Gy irradiation.However,the extent of depression of TCR ⁺,CD3⁺,CD4⁺CD8⁺,CD4⁺CD8^- and CD4^-CD8⁺ was diminished when a dose of 0.075 Gy was given 6 hours before 1.5 Gy.The results suggest that pre irradiation with 0.075 X-rays can significantly reduce the damage of thymocytes and the matured subsets induced by 1.5 Gy iradiation. Conclusion The results indicate that adaptive response of TCR⁺,CD3⁺,CD4 ⁺CD8 ⁺,CD4⁺CD8^- and CD4^-CD8⁺ subsets of thymocytes can be induced by whole body irradiation with 0.075 Gy X-rays.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭