| 苏旭,刘建香,张迎春,鞠桂芝,刘树铮.低剂量辐射诱导小鼠胸腺细胞及其亚组的适应性反应[J].中华放射医学与防护杂志,1999,19(3):174-176 |
| 低剂量辐射诱导小鼠胸腺细胞及其亚组的适应性反应 |
| Adaptive response of thymocytes and its subsets induced by low dose whole body irradiation with X-rays in mice |
| 投稿时间：1998-09-14 修订日期：1999-02-01 |
| DOI： |
| 中文关键词: 低剂量辐射 适应性反应 胸腺细胞亚组 |
| 英文关键词:Low dose irradiation Adaptive response Thymocytes subsets |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的 研究低剂量辐射预先照射以及随后大剂量照射后小鼠胸腺细胞T细胞受体(TCR)、CD3、CD4和CD8分子表达的变化。方法 TCR、CD3表达采用间接荧光流式细胞术检测, CD4、CD8表达采用双参数直接免疫荧光流式细胞术检测。结果 实验结果表明:单纯15GyX射线全身照射后TCR, CD3阳性细胞数以及CD4-D8-, CD4+CD8+, CD4+CD8-和CD4-CD8+细胞数明显减少, 当1.5GyX射线全身照射前6小时预先照射0.075Gy时, 可明显减轻其后15Gy照射对TCR+, CD3+, CD4+CD8+, CD4+CD8-和CD4-CD8+的损伤作用。表现为各亚组细胞数显着高于单纯1.5Gy照射组。CD4-CD8-亚组的细胞数无明显变化。结论 0.075GyX射线全身照射能够诱导胸腺细胞TCR+, CD3+, CD4+CD8+, CD4+CD8-和CD4-CD8+亚组细胞的适应性反应。 |
| 英文摘要: |
| Objective To study the changes in expression of TCR,CD3 CD4 and CD8 molecules on thymocytes following irradiation with low dose X-rays before a challenge dose in mice. Methods Kunming mice were irradiated with 0.075 Gy (conditioning dose) 6 hours before 1.5 Gy irradiation (challenge dose).The expression of TCR,CD3,CD4 and CD8 were analyzed by flow cytometry 18 hours after 1.5 Gy irradiation. Results It was found that the TCR+,CD3+,CD4-CD8+,CD4+CD8- and CD4-CD8 + positive cells were decreased significantly after only 1.5 Gy irradiation.However,the extent of depression of TCR +,CD3+,CD4+CD8+,CD4+CD8- and CD4-CD8+ was diminished when a dose of 0.075 Gy was given 6 hours before 1.5 Gy.The results suggest that pre irradiation with 0.075 X-rays can significantly reduce the damage of thymocytes and the matured subsets induced by 1.5 Gy iradiation. Conclusion The results indicate that adaptive response of TCR+,CD3+,CD4 +CD8 +,CD4+CD8- and CD4-CD8+ subsets of thymocytes can be induced by whole body irradiation with 0.075 Gy X-rays. |
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