| 罗灿,苏旭,付士波,等.X射线全身照射对小鼠脾细胞转铁蛋白受体表达的影响[J].中华放射医学与防护杂志,1999,19(2):119-121.LUO Can,SU Xu,FU Shibo,et al.Effects of whole body irradiation with X-rays on expression of TfR in splenocytes of mice[J].Chin J Radiol Med Prot,1999,19(2):119-121 |
| X射线全身照射对小鼠脾细胞转铁蛋白受体表达的影响 |
| Effects of whole body irradiation with X-rays on expression of TfR in splenocytes of mice |
| 投稿时间：1998-05-06 修订日期：1998-09-01 |
| DOI： |
| 中文关键词: 转铁蛋白受体 脾细胞 X射线 全身照射 |
| 英文关键词:Transferrin receptor Splenocytes X-rays Whole body irradiation |
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| 中文摘要: |
| 目的 转铁蛋白受体(TfR)是T淋巴细胞受抗原或丝裂原刺激后表达在细胞表面的重要受体之一,与T细胞的活化和增殖有着密切关系。目前尚未见国内外有关TfR辐射效应研究的报道。研究大剂量电离辐射后TfR表达的变化及作用,对阐明辐射抑制机体免疫功能的机制有着重要意义。方法 本文采用流式细胞术的方法观察了不同剂量X射线全身照射对小鼠脾细胞TfR表达的影响以及4GyX射线全身照射后不同时间TfR表达的变化。结果 0.5～6GyX射线全身照射后24小时,脾脏TfR阳性细胞数从1Gy开始随照射剂量的增加而下降,且呈明显的剂量依赖性。4GyX射线全身照射后12～72小时,脾脏TfR阳性细胞数于照后12小时开始下降,24、48小时降至最低值(P<0.05,P<0.01),72小时开始回升。结论 大剂量电离辐射抑制小鼠脾细胞TfR的表达,并有明显剂量依赖性；TfR表达的下降可能与辐射抑制IL -2的分泌和IL -2受体的表达有关。 |
| 英文摘要: |
| Objective Transferrin receptor(TfR) is one of important membrane receptors expressed in T cells stimulated with antigens or mitogens and is closely related with the activation and proliferation of T lymphocytes. Researches on radiation effects of TfR expression have not been reported yet. Studying the change and function of TfR expression after high dose ionizing radiation is important for clarifying the mechanism of ionizing radiation inhibiting immune function. Methods TfR expressions in lymphocytes in mouse spleens in different doses and at different time after the whole body irradiation (WBI) with X-rays were examined using flow cytometry. Results At 24 hours after the WBI with 0.5-6 Gy X-rays,TfR positive splenocytes decreased with the increasing irradiation dose beginning at 1Gy and showed dose dependent reduction. From 12 to 72 hours after WBI with 4 Gy X-rays TfR positive splenocytes started reducing at 12 hours after WBI and reached the nadir values at 24 hours and 48 hours (P<0.05,P<0.01),and began to recover from 72 hours after WBI. Conclusione High dose ionizing radiation inhibits the TfR expression in splenocytes of mice and there is an obvious dose dependency. The decrease of TfR expression may be related to the inhibition of IL-2 production and IL-2 receptor expression in cells by radiation. |
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