| 崔晓利,徐波,王争,等.高-低氧全肺照射对小鼠肺纤维化的影响[J].中华放射医学与防护杂志,1999,19(2):116-118.CUI Xiaoli,XU Bo,WANG Zheng,et al.Effect of irradiation in high and low oxygen on mouse lung fibrosis[J].Chin J Radiol Med Prot,1999,19(2):116-118 |
| 高-低氧全肺照射对小鼠肺纤维化的影响 |
| Effect of irradiation in high and low oxygen on mouse lung fibrosis |
| 投稿时间：1998-07-14 修订日期：1998-10-26 |
| DOI： |
| 中文关键词: 高-低氧放疗 肺纤维化 转化生长因子TGF-β1 RT-PCR |
| 英文关键词:Irradiation in HO+LO Lung fibrosis TGF-β1 RT-PCR |
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| 中文摘要: |
| 目的 以小鼠肺组织中TGF-β1含量变化为指标,观测高 低氧(HO+LO)照射对肺晚期损伤的生物学效应。方法 雄性C57BL小鼠,13Gy全肺一次照射,照射同时分别吸入空气、高氧(95%O2+5%CO2)或高 低氧(10%O2+5%CO2+85%N2),照射后不同时间取肺组织HE染色、Mason染色、V-G-染色观察肺纤维化形成。RT PCR方法测定肺组织内TGF-β1的表达。结果 照射后8个月肺组织局部灶性纤维化形成。高氧组肺纤维化面积大于空气组及HO+LO组,两者相比,差异具有显着性；HO+LO组纤维化面积虽略高于空气组,但差异无显着性。照射后36小时,TGF-β1表达下降,照射后2个月,TGF-β1表达升高,至照射后8个月,略呈下降趋势,但仍高于正常对照。结论 低氧的加入降低单纯高氧导致的正常组织损伤,HO+LO照射不增加肺的晚期损伤,TGF-β1的表达在肺纤维化形成过程中起重要作用。 |
| 英文摘要: |
| Objective To study the effect of irradiation in high and low oxygen (HO+LO) on the development of mouse lung fibrosis. Methods C57 BL mice were divided into three groups of irradiation:in air,HO(95%O2+5%CO2),and HO+LO(10%O2+5%CO+85%N2). Using the lungs of C57 BL mice,the histological changes were observed up to 8 months after whole thorax irradiation. Expression of TGF-β1 mRNA was evaluated by RT-PCR. Results The area of fibrosis was 2213 scale/lung (in air),2310 scale /lung(in HO+LO) and 6460 scale/lung(in HO),respectively. The expression of TGF-β1 decreased at 36 hours after whole thoracic irradiation,and increased after 2 months,then showed a modest elevation after 8 months when lung fibrosis appeared. Conclusione This study suggests in that irradiation that HO+LO can reduce the lung damage due to irradiation HO. TGF-β1 may play a role in the development of chronic fibrosis. |
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