| 岳井银,刘炳辰,吴红英,等.137Cs γ射线诱发CHL细胞遗传不稳定性[J].中华放射医学与防护杂志,1999,19(2):112-115.YUE Jingyin,LIU Bingchen,WU Hongying,et al.Genomic Instability induced by 137 Cs γ-ray irradiation in CHL surviving cells[J].Chin J Radiol Med Prot,1999,19(2):112-115 |
| 137Cs γ射线诱发CHL细胞遗传不稳定性 |
| Genomic Instability induced by 137 Cs γ-ray irradiation in CHL surviving cells |
| 投稿时间：1998-05-10 修订日期：1998-10-05 |
| DOI： |
| 中文关键词: 基因组不稳定性 HGPRT位点突变 微核 细胞凋亡 |
| 英文关键词:Genomic instability Mutationhgprt locus Micronuclei Apoptosis |
| 基金项目:国家自然科学基金 |
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| 摘要点击次数: 3226 |
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| 中文摘要: |
| 目的 研究辐射诱发的CHL细胞遗传不稳定性在基因、染色体及细胞水平上的传递及表达情况。方法 不同剂量一次照射CHL细胞,于不同时间点分析受照存活细胞的HGPRT位点突变、微核及细胞凋亡。结果 CHL细胞照射3Gy后53天,其后代仍表达出显着升高的HG PRT位点突变率。细胞照射3Gy后6小时的双核细胞微核率达(41.51±3.61)%,3天后下降为(14.47±2.39)%,56天后为(10.51±0.87)%,显着高于对照组(P<0.01);而受照细胞子代双核率无显着变化,接种效率明显降低(3Gy剂量以上P<0.01).细胞受照射3、4、6、9、10Gy后,第2天细胞凋亡率达最大值,10Gy剂量组为(24.90±6.72)%,之后各剂量组细胞凋亡率迅速下降,但直到照射后12天细胞凋亡率仍维持在10%左右,显着高于对照组(P<0.01).结论 辐射诱发的细胞基因组不稳定性可以表现在基因、染色体及细胞等不同水平上,推测在其产生和传递过程中可能伴随着某些基因的改变。 |
| 英文摘要: |
| Objective To study in parallel several possible manifestations of instability of surviving CHL cells after irradiation,namely the frequencies of mutation at locus,micronuclei and apoptosis.Methods The frequencies of mutation at hgprt locus,micronuclei and apoptosis were assayed at various times in surviving cells irradiated with γ-rays.Results The surviving cells showed a persistently increased frequency of mutation at the hgprt locus after irradiation until 53 days.Mutant fraction as high as 10-4 was scored,tens of times higher than those assayed in control cells studied in parallel.The frequency of binucleated cells with micronuclei determined within 24 hours after irradiation increased with dose and reached a peak value of (26.58±2.48)% at 3Gy,decreasing at higher doses to a plateau around 20%.The micronucleus frequency decreased steeply to about(14.47±2.39)% within the first 3 days post irradiation,and fluctuated at around 10% up to 56 days post irradiation.The delayed efficiency of irradiated cells was significantly decreased.The frequency of apoptosis peaked about(24.90±4.72)% at 10 Gy 48 h post irradiation(γ-ray dose between 3 10 Gy)and then decreased to about 12% within 3 days.It was significantly higher than in control cells until 14 days.Conclusiones It shows that genomic instability induced by radiation can be transmitted to the progeny of surviving cells and may take many forms of expression such as lethal mutation,chromosome aberrations,gene mutation,etc. |
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