| 傅海青,鞠桂芝.X射线全身照射后脾细胞内[Ca2+)]i对Con A反应性的变化[J].中华放射医学与防护杂志,1999,19(1):27-29 |
| X射线全身照射后脾细胞内[Ca2+)]i对Con A反应性的变化 |
| Effect of whole body irradiation with X rays on [Ca2+]i response to Con A in splenocytes |
| 投稿时间：1998-09-22 修订日期：1998-12-20 |
| DOI： |
| 中文关键词: 电离辐射 脾细胞 Fura-2/AM 细胞内游离钙离子 |
| 英文关键词:Ionizing radiation Splenocytes Fura 2AM Intracellular free Ca 2+ |
| 基金项目:国家自然科学基金 |
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| 摘要点击次数: 2928 |
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| 中文摘要: |
| 目的 研究电离辐射对ConA激活的脾细胞内游离钙离子[Ca2+]i)动员的影响, 以探讨电离辐射免疫抑制作用的发生机理。方法 采用Fura2/AM双波长荧光测定法检测了X射线全身照射后的小鼠脾细胞内[Ca2+]i对ConA反应性的变化。结果 小鼠接受0、0.5、1、2、4和6Gy吸收剂量的X射线全身照射后24小时, 脾细胞内[Ca2+]i对ConA反应性呈剂量依赖性下降, 表现为[Ca2+]i上升幅度减小、上升至峰值时间延长。2GyX射线全身照射后的时程观察表明, 照后2小时细胞内[Ca2+]i对ConA反应性开始下降, 24小时达最低点, 照后5天才略有回升。结论 电离辐射所致免疫功能抑制与其抑制淋巴细胞内[Ca2+]i动员等信息传递过程有关。 |
| 英文摘要: |
| Objective To elucidate the possible mechanism of immunodepression following whole body irradiation (WBI) with X rays,intracellular free Ca 2+ ([Ca2+]i) response to Con A in splenocytes of mice after WBI was studied. Method Fluorescent indicator fura-2 was used to assess the changes of [Ca2+]i response to Con A in splenocytes of Kunming mice following WBI with X-rays. Results WBI with 0,0.5,1,2,4, and 6 Gy X-rays,respectively,could inhibit dose dependently mitogen Con A-stimulated [Ca2+]i elevation and prolong the time of Con A-stimulated [Ca2+]i elevation to a peak level in splenocytes of mice 24h after irradiation.Time course of 2 Gy WBI with X-rays indicated that inhibition of Con A-stimulated [Ca 2 ] i increase in splenocytes began at 2h,reached the lowest value at 24h,and slightly recovered at 5d after WBI. Conclusion Immunodepressive effect of ionizing radiation is associated with inhibition of [Ca2+]i mobilization in T lymphocytes. |
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