| 陈幸华,罗成基,郭朝华,王苹.放烧复合伤对小鼠骨髓基质祖细胞体外集落生成能力的影响[J].中华放射医学与防护杂志,1999,19(1):22-23 |
| 放烧复合伤对小鼠骨髓基质祖细胞体外集落生成能力的影响 |
| Capacity of bone marrow colony forming unit fibroblasts in vitro from mice with combined radiation burn injury |
| 投稿时间：1998-06-17 修订日期：1998-10-27 |
| DOI： |
| 中文关键词: 放射损伤 烧伤 放烧复合伤 骨髓 基质细胞 小鼠 |
| 英文关键词:Radiation injury Burn Combined radiation burn injury Bone marrow Stromal cells Mice |
| 基金项目:全军“八五”攻关基金 |
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| 中文摘要: |
| 目的 观察放烧复合伤对小鼠骨髓基质细胞集落形成能力(CFU-F)的影响。方法 采用Dexter型方法, 检测经5.0Gyγ射线放射损伤、15%Ⅲ度体表面积烧伤和放烧复合伤小鼠于伤后不同时间骨髓基质细胞集落形成能力。结果 单放组和复合伤组于伤后各时相点均显着低于正常组和单烧组；单烧组除伤后第3天低于正常组外(P<0.05), 至伤后第14天与正常组差异无显着性。单放组骨髓基质细胞在伤后3天和7天经21天培养时呈小团片状生长, 至培养第28天时仍未长满培养皿底。结论 单放组和复合伤组骨髓基质细胞受损后恢复缓慢；放烧复合伤对骨髓基质细胞的损伤具有放射损伤为主的特点。 |
| 英文摘要: |
| Objective To investigate the capacity of bone marrow colony forming unit fibroblasts (CFU F) from mice with combined radiation burn injury. Methods Mice were treated with 5.0 Gy γ-ray radiation alone, 15% total body surface area (TBSA) Ⅲ° burn alone or combined radiation burn.The numbers of CFU-Fs were assayed by Dexter′s method. Results The numbers of CFU-Fs from mice with radiation and combined radiation burn injury were significantly decreased,compared with those of controls and mice with burn injury alone ( P <0.05-0.01). Conclusion The results reveal that the repairing process of bone marrow stromal cells from mice with radiation injury and combined radiation-burn injury is slow,and the combined radiation-burn injury inflicted on the stromal cells possesses the characteristic of radiation injury. |
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