| 徐辉,程天民,粟永萍,等.全身辐射对小鼠小肠上皮内淋巴细胞功能影响的实验研究[J].中华放射医学与防护杂志,1999,19(1):18-21.XU Hui,CHENG Tianmin,SU Yongping,et al.Effects of total body irradiation on functions of small intestinal intraepithelial lymphocytes[J].Chin J Radiol Med Prot,1999,19(1):18-21 |
| 全身辐射对小鼠小肠上皮内淋巴细胞功能影响的实验研究 |
| Effects of total body irradiation on functions of small intestinal intraepithelial lymphocytes |
| 投稿时间：1998-07-17 修订日期：1998-10-15 |
| DOI： |
| 中文关键词: 辐射 粘膜免疫 小肠上皮内淋巴细胞 |
| 英文关键词:Irradiation Mucosal immunity Intestinal intraepithelial lymphocytes |
| 基金项目:全军“九五”指令性攻关课题基金 |
|
| 摘要点击次数: 3424 |
| 全文下载次数: 1964 |
| 中文摘要: |
| 目的 探讨辐射对粘膜免疫的影响。方法 小鼠全身照射, 吸收剂量为3、8、12Gy, 然后分离培养全小肠上皮内淋巴细胞(IEL), 并对其数量、细胞增殖活力、细胞毒力、TNF-α和TGF-β分泌水平进行检测。结果 ①全身照射后使IEL数量减少, 3种剂量均呈现出伤后8小时下降, 48～72小时达最低值, 7天回升, 至15天尚未恢复正常水平；各剂量组间差异无显着性, 但8Gy组较3Gy组恢复慢, 12Gy组未待回升动物已死亡；②全身照射后IEL功能受抑, 表现为增殖活力和细胞毒力下降, 其变化过程和数量减少相一致；③全身照射后IEL分泌TNF-α和TGF-β增多, 也呈现为8小时升高, 48～72小时达高峰, 15天仍未下降至正常值的过程。结论 放射损伤导致IEL数量减少及功能降低, 造成肠粘膜免疫功能的下降, 从而共同导致肠道屏障功能的削弱。 |
| 英文摘要: |
| Objective To explore the characteristics of intestinal mucosal immunity after gamma irradiation. Methods The number, proliferation activity, cytotoxic activity of small intestinal intraepithelial lymphocytes (IELs),and the TNF-α and TGF-β concentrations in supernatant of cultured IELs were studied using IELs freshly isolated from whole small intestine of Kunming strain mice after 3,8 and 12 Gy total body 60 Co γ irradiation. Results (1)The number of IELs in small intestinal mucosa of all irradiated mice significantly decreased at 8h,reaching the lowest level at 48-72h post irradiation,then began to rise,but it still did not return to its normal level on day 15.(2)The proliferation activity and cytotoxic activity of IELs isolated from irradiated mice were reduced sharply.They followed the same pattern of decreasing at 8h,reaching the lowest level at 48-72h post irradiation,then began to rise,but it did not return to their normal levels on day 15.(3)The TNF-α and TGF-β concentrations in supernatant of cultured IELs isolated from irradiated mice were elevated at 8h,reaching their peak at 48-72h. Conclusion The decrease in number and important functions of IELs is one of the factors damaging the intestinal mucosal immunity barrier after total body irradiation. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|