| 朱茂祥,郭仁锋,杨陟华,等.肿瘤坏死因子-α对α粒子致叙利亚地鼠胚胎细胞恶性转化的促进作用[J].中华放射医学与防护杂志,1999,19(1):6-10.ZHU Maoxiang,GUO Renfeng,YANG Zhihua,et al.Promotion of TNF α on malignant transformation of Syrian hamster embryo cells irradiated with α particles[J].Chin J Radiol Med Prot,1999,19(1):6-10 |
| 肿瘤坏死因子-α对α粒子致叙利亚地鼠胚胎细胞恶性转化的促进作用 |
| Promotion of TNF α on malignant transformation of Syrian hamster embryo cells irradiated with α particles |
| 投稿时间：1998-06-25 修订日期：1998-08-16 |
| DOI： |
| 中文关键词: TNF-α SHE α粒子照射 细胞转化 蛋白质酪氨酸磷酸化 |
| 英文关键词:Tumor necrosis factor α Syrian hamster embryo cells Alpha particle irradiation Cell transformation Protein tyrosine phosphorylation |
| 基金项目:国家自然科学基金;军事医学科学院青年基金 |
|
| 摘要点击次数: 3400 |
| 全文下载次数: 2026 |
| 中文摘要: |
| 目的 阐明辐射致癌过程中肿瘤坏死因子-α(TNF-α)的作用及蛋白质酪氨酸磷酸化调节机理。方法 以0.5Gyα粒子照射的叙利亚地鼠胚胎(SHE)细胞为靶细胞, 观察细胞生长曲线、转化频率(TF)、软琼脂克隆形成率(CFE)和裸鼠致瘤性, 并测定细胞中蛋白质酪氨酸激酶和酪氨酸磷酸酶活性。结果 单纯0.5Gyα粒子照射或TNF-α不能使SHE细胞发生恶性转化, 600U/mlTNFα处理0.5Gyα粒子照射的SHE细胞后, 细胞获得了长期传代的能力, TF、CFE以及细胞中蛋白质酪氨酸磷酸化水平均明显增高, 裸鼠致瘤性试验为阳性。结论 TNF-α对α粒子致SHE细胞转化有显着促进作用, 且该过程中可能涉及蛋白质酪氨酸磷酸化的调控机制。 |
| 英文摘要: |
| Objective To illustrate the role of tumor necrosis factor α(TNF-α) in radiation induced cancer and regulatory mechanism of protein tyrosine phosphorylation.Methods Taking Syrian hamster embryo cells exposed to 0.5 Gy α-particles as the target, an array of biological indicators such as cell growth curve, transformation frequency (TF), colony formation efficiency(CFE) and tumor formation in nude mice were observed, and the activities of protein tyrosine kinases and protein tyrosine phosphatases were measured.Results Neither 0.5 Gy α-particle irradiation nor TNF α alone could induce transformation of SHE cells morphologically, but the TF, CFE and levels of protein tyrosine phosphorylation were obviously increased in SHE cells treated with 600 U/ml TNF-α after exposure to 0.5Gy α-particles, and malignant transformation was proved by tumorigenicity assays.Conclusion TNF-α promotes significantly the transformation of SHE cells induced by α-particles, and protein tyrosine phosphorylation is probably involved in regulation of the process. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |