| 宋良文,马宪梅,崔雪梅,白蕴红,王德文.60Co-γ射线照射对大鼠肺一氧化氮合酶和内皮素系统基因表达的影响[J].中华放射医学与防护杂志,1998,18(6):396-398 |
| 60Co-γ射线照射对大鼠肺一氧化氮合酶和内皮素系统基因表达的影响 |
| Effect of 60Co γ-rays on gene expression of nitric oxide synthase and endothelin system in rat lung. |
| 投稿时间：1997-08-12 修订日期：1997-12-22 |
| DOI： |
| 中文关键词: 辐射 基因表达 一氧化氮合酶 内皮素 肺 |
| 英文关键词:Radiation Gene expression Nitric oxide synthase Endothelin Lungs |
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| 中文摘要: |
| 目的 探讨一氧化氮合酶(nitric oxide synthase,NOS)和内皮素(endothelin,ET)及ET转换酶(endothelin converting enzyme,ECE)等在受照射肺中的基因表达变化,揭示其在肺纤维化发生中的参与作用。方法 用60Co对大鼠肺进行30Gy照射,照后4,8,24天提取总RNA,分别做RT-PCR;对照射后1,3,5个月肺组织进行ET肽的Westernblot和NOS-mRNA原位杂交。结果 照射后肺NOSmRNA转录减弱,ETmRNA增强,ECEmRNA在第8和24天增强,ETB-R mRNA在第24天转为阴性。照射后1,3,5月肺组织ET肽合成增加。照射后5个月NOSmRNA原位杂交呈阴性。结论 ET和NO之间平衡失调可能与肺纤维化发生有关。 |
| 英文摘要: |
| Objective Gene expressions of nitric oxide synthase (NOS) and endothelin (ET) as well as ET converting enzyme (ECE) in rat lung after irradiation and their roles in genesis of pulmonary fibrosis were investigated. Methods Rat lung was irradiated with 30Gy of 60Coγ-rays and pulmonary total RNA was extracted at days 4, 8 and 24 after irradiation, respectively.RT PCR was made.Western blot of ET peptide in lung tissues was performed at 1, 3 and 5 months after irradiation.Meanwhile in situ hybridization of NOS mRNA in rat pulmonary tissues was made at 5 months after irradiation. Results NOS mRNA transcription in pulmonary tissues turned into negative;ET mRNA transcription displayed strong positivity; positive ECE mRNA transcription appeared at days 8 and 24 after irradiation and ETB-R mRNA transcription turned to negative at day 24 after irradiation.Western blot of ET in plumonary tissue all displayed positive at 1, 3, and 5 months after irradiation, especially at 1 month.In situ hybridization of NOS mRNA was negative at 5 moths after irradiation. Conclusion Dysregulation of balance between ET and NO may be involved in genesis of radiation pulmonary fibrosis. |
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