| 刘永彪,苏燎原,孔向蓉.榄香烯对小鼠脾淋巴细胞亚群及其调节的辐射保护效应[J].中华放射医学与防护杂志,1998,18(6):386-389 |
| 榄香烯对小鼠脾淋巴细胞亚群及其调节的辐射保护效应 |
| Radioprotective effects of elemene on spleen lymphocyte subsets of mice and its regulation. |
| 投稿时间：1997-12-03 修订日期：1998-05-11 |
| DOI： |
| 中文关键词: 榄香烯 淋巴细胞亚群 细胞膜 DNA 自由基 过氧化反应 |
| 英文关键词:Elemene Lymphocyte subsets Cell membrane DNA Free radicals Peroxidation |
| 基金项目: |
|
| 摘要点击次数: 2486 |
| 全文下载次数: 1646 |
| 中文摘要: |
| 目的 探讨榄香烯对受照小鼠脾淋巴细胞及亚群间调节作用的保护效应和剂量范围。方法 应用单克隆抗体铺皿法分离受60Coγ射线分次照射的小鼠脾脏L3,T4,Lyt2和B细胞,分别测定榄香烯对以上三种亚群细胞和L3,T4,Lyt2对B细胞3H-TdR掺入的影响,同时测定SOD活力和LPO含量。结果 榄香烯提高了受照2.5Gy(0.5Gy×5d)小鼠脾淋巴细胞亚群的辐射抗性,增强了L3T4对B细胞的辅助调节作用,提高了脾脏SOD活力和降低LPO含量。而对受照4Gy(0.8Gy×5d)和5Gy(0.5Gy×10d)组没有观察到这种效应。结论 在一定剂量范围,榄香烯通过清除自由基减少靶分子的损伤。 |
| 英文摘要: |
| Objective To explore the radioprotective effcets of elemene on spleen lymphocyte subsets of mice and the relationship between dose and effect, and its regulation.Methods The spleen lymphocytes in mice were separated into L3T4, Lyt2 and B cells after fractional irradiation by a panning method with monoclonal antibodies, and the effects of elemene on function and regulation of lymphocyte subsets were studied by using 3 H TdR incorporation. The SOD activety and LPO content were measured as well.Results Elemene could increase the radiation resistance of lymphocyte subsets of 2.5Gy(0.5Gy×5d) group, enhance the auxiliary effects of L3T4 on B cell, increase spleen SOD activity and decrease LPO content. Not such effect were observed in 4Gy(0.8Gy×5d) and 5Gy(0.5Gy×10d) gyoups.Conclusion The mechanism of protection of elemene in limied dose range may be due to its scavenging free radicals and relieving the damage of target cells. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|