| 李夏新,洪平,程少杰,F.Leteurtre.γ射线照射后造血细胞端粒酶活性变化[J].中华放射医学与防护杂志,1998,18(6):377-380 |
| γ射线照射后造血细胞端粒酶活性变化 |
| Change of telomerase activity in gamma irradiated hematopoietic cells. |
| 投稿时间：1997-04-03 修订日期：1997-11-24 |
| DOI： |
| 中文关键词: γ射线 造血细胞 端粒酶 细胞周期 |
| 英文关键词:Gamma rays Hematopoietic cells Telomerase Cell cycle |
| 基金项目:军队“八·五”青年基金 |
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| 中文摘要: |
| 目的 以KGla和CEM两个白血病细胞株为模型,探讨γ射线照射后造血细胞中端粒酶的变化。方法 细胞经照射后用3H-胸腺嘧啶渗入法测定细胞增殖能力,Kim氏TRAP方法分析端粒酶活性,并采用2次胸腺嘧啶使细胞同步化,BrdU渗入法分析细胞同步化结果及照射后细胞周期分布。结果 γ射线照射后造血细胞能增加端粒酶活性,在0～3Gy照射后酶活性逐渐上升,3Gy达高峰,在时间动态变化上,照射后8～24小时酶活性最高,此后开始下降,此时在细胞周期分布上有G2/M期细胞堆积,然而使细胞同步化后端粒酶活性在细胞各期无明显变化。结论 造血细胞的端粒酶活性在照射后的上调反应,可能与DNA的修复、稳定断裂染色体有关。 |
| 英文摘要: |
| Objective Using two hematopoietic cell lines, KGla and CEM, to investigate the variation of telomerase activity after γ-irradiation.Methods After irradiation, cell proliferation was measured by thymidine incorporation and telomerase activity of cells was analysed by Kim's TRAP assay. Cell synchronization was achieved after two-step thymidine blocks, and cell cycle was examined by BrdU incorporation technique for verifying cell cycle distribution after irradiation and cell synchronization.Results Telomerase activity increased after irradiation of between 0 and 3Gy in a dose-dependent manner, reaching the maximum at 3 Gy. The increase of telomerase activity was nearly to its maximum 8h after irradiation, the peak being observed at around 24h. Although this kinetics partly correlated with cell redistribution into the G2/M phase of the cell cycle, telomerase activity did not show significant variation over the cell cycle.Conclusion The up-regulation of telomerase in hematopoietic cell lines after γ-irradiation may suggest the involvement of telomerase in DNA repair and chromosome healing. |
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