| 谢风,苏旭,刘树铮.不同剂量X射线全身照射后胸腺细胞游离钙浓度的变化时程[J].中华放射医学与防护杂志,1998,18(5):351-353 |
| 不同剂量X射线全身照射后胸腺细胞游离钙浓度的变化时程 |
| Time course of intracellular free Ca2+ concentration changes in thymocytes after whole body irradiation with X-rays |
| 投稿时间：1997-01-20 修订日期：1997-06-09 |
| DOI： |
| 中文关键词: 低剂量辐射 [Ca2+]i 信号传递 Fura-2AM |
| 英文关键词:Low dose radiation Intracellular free Ca2+ concentration Signal transduction Fluorescence probe |
| 基金项目:国家自然科学基金 |
|
| 摘要点击次数: 2649 |
| 全文下载次数: 670 |
| 中文摘要: |
| 目的 研究X射线全身照射后胸腺细胞游离钙浓度的变化时程, 以揭示低剂量辐射免疫增强效应的分子机理。方法 应用Fura-2AM荧光探针, 采用荧光分光光度法检测了小鼠胸腺细胞内游离钙浓度。结果 75mGy全身照射后可使细胞内游离钙浓度增高, 24小时达峰值, 72小时回降至对照水平。结论 低剂量辐射可促进T淋巴细胞信号传递。 |
| 英文摘要: |
| Objective The purpose of the present study was to look at the possible influence of whole body irradiation (WBI) with X-rays on the intracellular free Ca2+ concentration [Ca2+]i in thymocytes and to elucidate the possible molecular mechanism of immunoenhancement following low dose radiation (LDR).Method [Ca2+]i was assayed by fluoresence spectrophotometry after loading the thymocytes with a fluorescence probe (Fura-2AM).Result It was found that [Ca2+]i increased after WBI with 75mGy X-rays, reaching its peak at 24h and returning to control level within 72h.Conclusion The results indicate that LDR can promote signal transduction of T lymphocytes. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |