| 田琼,张绍章,蒲勤,等.重组人骨形成蛋白-2成熟肽对小鼠辐射损伤的治疗作用及其机理的探讨[J].中华放射医学与防护杂志,1998,18(4):253-255.Tian Qiong,Zhang Shaozhang,Pu Qin,et al.Effect and mechanism of rhBMP-2m on acute hemopoietic radiation injury in mice[J].Chin J Radiol Med Prot,1998,18(4):253-255 |
| 重组人骨形成蛋白-2成熟肽对小鼠辐射损伤的治疗作用及其机理的探讨 |
| Effect and mechanism of rhBMP-2m on acute hemopoietic radiation injury in mice |
| 投稿时间：1997-11-05 修订日期：1998-03-03 |
| DOI： |
| 中文关键词: 重组人骨形成蛋白-2成熟肽 放射损伤 粒巨细胞集落形成单位 CFU-GM |
| 英文关键词:rhBMP-2m Acute radiation injury Bone marrow form CFU-GM |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨重组人骨形成蛋白-2成熟肽(rhBMP-2m)对小鼠经6.5～7.5Gyγ射线全身照射引起的放射损伤的治疗作用及其机理。方法 小鼠照射后1～6天于腹腔内注射rhBMP-2m生理盐水溶液, 每天一次, 每次剂量为0.5～2.0mg.观察动物的30天存活情况及几种造血指标。结果 治疗组的30天存活率, 平均存活时间, CFU-GM集落形成数, 骨髓细胞DNA含量, 骨髓有核细胞数, 脾结节形成数, 脾体比及外周血白细胞数均较照射对照组增加, 差异有非常显着性(P<0.01).结论 rhBMP-2m对小鼠的放射损伤有治疗作用, 其机理可能同诱导造血因子释放, 改善造血微环境, 促进造血功能恢复有关。 |
| 英文摘要: |
| Objective To study the effect of rhBMP-2m on irradiated mice.Method 104 male BALB/c mice were randomized to several groups and exposed to 6.5-7.5 Gy whole body 60Co γ rays.After irradiation a dose from 0.5 to 2.0 mg rhBMP-2m in saline were injected i.p. once daily in 1 6 consecutive days, and the animals' 30 day survival was scored.Some indices with relation to hematogenesis were observed as well at ninth day after irradiation.Results It was showed that the 30 day survival, mean survival time, count of bone marrow cells, DNA content of bone marrow cells, count of CFU-GM on agar culture, peripheral blood WBC count, spleen/body weight ratio and number of spleen colony formation were all significantly higher in 2.0 mg rhBMP-2m treated groups than those in the corresponding irradiated controls (P<0.01).Conclusion The beneficial effect of rhBMP-2m on the hemaotogenesis in irradiated mice can be ascribed supposedly to that the rhBMP-2m may induce the release of hematopoietic growth factor(s) in irradiated animal body, or it may act as a hematopoietic growth factor itself, or it may induce a hematopoietic microenvironment to support the growth of stem cells. |
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