| 项莺松,杨如俊,闵锐,等.胎肝基质细胞强化骨髓移植治疗急性放射病[J].中华放射医学与防护杂志,1998,18(4):249-252.Xiang Yingsong,Yang Rujun,Min Rui,et al.Therapy for acute radiation disease with bone marrow transplantation enhanced by fetal liver stromal cells in mice[J].Chin J Radiol Med Prot,1998,18(4):249-252 |
| 胎肝基质细胞强化骨髓移植治疗急性放射病 |
| Therapy for acute radiation disease with bone marrow transplantation enhanced by fetal liver stromal cells in mice |
| 投稿时间：1997-09-10 修订日期：1997-10-27 |
| DOI： |
| 中文关键词: 骨髓移植 胎肝基质细胞 龛位 急性放射病 急性移植物抗宿主病 |
| 英文关键词:Bone marrow transplantaition Niche Fetal liver stromal cells Graft versus host disease Acute radiation disease |
| 基金项目:国家自然科学基金 |
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| 摘要点击次数: 5549 |
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| 中文摘要: |
| 目的 为进一步提高骨髓移植效果。方法 以昆明种小鼠急性放射病为模型, 进行了胎肝基质细胞强化同种异基因骨髓移植, 观察了小鼠造血重建、急性移植物抗宿主病(GVHD)及小鼠存活率。结果 胎肝基质细胞移植可强化骨髓移植效果, 与单纯骨髓移植组比较, 于照射后第11天小鼠外周血白细胞、骨髓有核细胞计数, CFU-E、CFU-GM、CFU-F、CFU-S回升较快, CFU-F已达正常, 于照射后第17天造血得以重建；GVHD较轻, 存活率显着提高, 达60%.结论 胎肝基质细胞改善了造血微环境, 可能既改善了"龛位", 又增加了"龛位"使干细胞更多的植入, 促进了造血重建, 提高了骨髓移植效果, 是一种"种子与土壤"并重的移植新方法。 |
| 英文摘要: |
| Objective To enhance the grafting efficiency of bone marrow transplantation (BMT). Method The survival rate of mice, hematopoietic reconstitution, and reduction of acute graft versus host disease (GVHD) were studied in an acute radiation disease model of Kunming mice by a new method of BMT enhanced by addition of fetal liver stromal cells (BMT-FLSt).Results WBC and nucleated cell counts and indexes of restored to normal CFU-E, CFU-GM, CFU-S, especially CFU-F, in BMT-FLSt group were significantly higher than those in the group with BMT alone 11 days after irradiation.And all those indxes in BMT-FLSt group returned to normal 17 days after irradiation.The survival rate of mice in BMT-FLSt group (60%) was also significantly higher than that in the group with BMT alone (30%), and the degree of GVHD in BMT-FLSt group was slighter than that in the group with BMT alone. Conclusion These data suggested that hematopoietic microenvironment could be improved, and "niches" could be both improved and increased by fetal liver stromal cell transplantation; so, fetal liver stromal cells could prompt hematopoietic reconstitution and enhance engrafting efficiency. |
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