| 丁桂荣,郭鹞.L-NAME对小肠上皮细胞辐射防护作用机理的探讨[J].中华放射医学与防护杂志,1998,18(3):156-158.Ding Guirong,Guo Yao.Effect of L-NAME on radiation induced IEC-6 cell injury[J].Chin J Radiol Med Prot,1998,18(3):156-158 |
| L-NAME对小肠上皮细胞辐射防护作用机理的探讨 |
| Effect of L-NAME on radiation induced IEC-6 cell injury |
| 投稿时间：1997-11-07 修订日期：1997-09-01 |
| DOI： |
| 中文关键词: 硝基左旋精氨酸甲基酯 小肠 上皮细胞 一氧化氮 辐射 |
| 英文关键词:L-NAME Small intestines Epithelial cells Nitric oxide Radiation |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨硝基左旋精氨酸甲基酯(L-NAME)对小肠上皮细胞辐射防护的机理。方法 实验用大鼠IEC-6细胞, 60Coγ射线照射；荧光分光光度法测定培养液上清中一氧化氮(NO)含量；NADPH黄递酶组化法(ND组化法)和免疫细胞化学法观察一氧化氮合酶(NOS)活性变化；MTT法检测细胞的生存力。结果 IEC-6细胞经射线照射后, 其培养液上清中NO含量明显增加, 细胞内结构型一氧化氮合酶(cNOS)活性升高, 诱导型一氧化氮合酶(iNOS)活性降低, L-NAME能明显提高细胞的生存力, NO底物-左旋精氨酸(L-Arg)部分逆转其作用。结论 NO参与了IEC-6细胞的辐射损伤, L-NAME对IEC-6细胞具有辐射防护作用。 |
| 英文摘要: |
| Objective To study the effect of L-NAME on radiation induced injury of small intestinal epithelial cells 6(IEC-6).Methods Rat IEC-6 cells were irradiated with 60Coγ rays.The content of nitric oxide(NO) in media supernatant of IEC-6 cells was measured by fluorometric method.Nitric oxide synthase (NOS) activity was observed by histochemistry of NADPHdiaphase and immunocytochemistry.Cell viability was evaluated by method of MTT.Results NOgeneration by IEC-6 cells was significantly increased after irradiation.The assay of cNOSactivity showed that it was stimulated.iNOSactivity was inhibited.L-NAME was able to increase cell viability which had been reduced by irradiation, but it was reversed partly by pretreatment with NOsubstrate L Arg.Conclusion NOwas involved in IEC-6 cell injury induced by radiation and L-NAME could provide protective effect on it. |
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