| 彭瑞云,王德文,熊呈琦,等.LACA小鼠骨髓辐射损伤后干细胞因子受体表达的免疫组化研究[J].中华放射医学与防护杂志,1998,18(3):153-155.Peng Ruiyun,Wang Dewen,Xiong Chengqi,et al.Immunohistochemical studies on expression of C kit in LACA mouse bone marrow after irradiation[J].Chin J Radiol Med Prot,1998,18(3):153-155 |
| LACA小鼠骨髓辐射损伤后干细胞因子受体表达的免疫组化研究 |
| Immunohistochemical studies on expression of C kit in LACA mouse bone marrow after irradiation |
| 投稿时间：1996-07-15 修订日期：1996-10-03 |
| DOI： |
| 中文关键词: 辐射 骨髓 细胞凋亡 C-kit 免疫组化 |
| 英文关键词:Radiation Bone marrow Apoptosis C kit Immunohistochemistry |
| 基金项目: |
| 作者 | 单位 | | 彭瑞云 | 100850 北京, 北京放射医学研究所 | | 王德文 | 100850 北京, 北京放射医学研究所 | | 熊呈琦 | 100850 北京, 北京放射医学研究所 | | 李延平 | 100850 北京, 北京放射医学研究所 | | 高亚兵 | 100850 北京, 北京放射医学研究所 | | 汪宝珍 | 100850 北京, 北京放射医学研究所 | | 崔玉芳 | 100850 北京, 北京放射医学研究所 | | 杨红 | 100850 北京, 北京放射医学研究所 | | 杨瑞彪 | 100850 北京, 北京放射医学研究所 | | 崔雪梅 | 100850 北京, 北京放射医学研究所 |
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| 中文摘要: |
| 目的 至今, 尚未见到采用免疫组化技术研究骨髓辐射损伤后干细胞因子受体(C-kit)表达及其在造血重建中的意义。方法 经60Coγ射线全身照射LACA小鼠, 于照射后4周内分批活杀动物, 采用光镜、电镜及LSAB免疫组化方法, 研究了辐射诱导的造血细胞凋亡及C-kit的表达及意义。结果 全身照射LACA小鼠时, 5.5Gy为其亚致死剂量, 此剂量照射小鼠可导致外周血白细胞及骨髓有核细胞数减少；骨髓明显损伤及损伤后重建现象；在照后1天内可使骨髓造血细胞发生凋亡；5.5Gy照射后6小时, C-kit蛋白于造血细胞浆内明显增多, 呈强阳性；照后10天, 骨髓造血细胞增生、恢复, C-kit蛋白于造血细胞膜表达明显增多, 呈强阳性。结论 C-kit表达与造血功能重建有关, 在早期造血功能重建中起重要调节作用。 |
| 英文摘要: |
| Objective To study the expression and significance of Ckit in irradiated bone marrow.Methods LACAmice were irradiated with 60Coγ rays, and sacrificed within 4 weeks after irradiation.By means of light microscopy, electron microscopy and immunohistochemical method of LSAB, the authors studied radiation induced apoptosis of hemopoietic cells and the expression of Ckit.Results Adose of 5.5Gy total body irradiation was a sublethal dose for LACAmouse, and induced decrease of WBCin blood and nucleated cells in bone marrow.The bone marrow was obviously injured and then reconstructed.Ckit protein was strongly positive in cytoplasma of hematopoietic cells 6 hours after radiation, while it was strongly positive at the membrane of hematopoietic cells 10 days after irradiation.Conclusion The hemopoietic cells developed apoptosis within 1 day after irradiation.The expression of Ckit was related to the reconstruction of hematopoiesis, and played an important role in the early hematopoiesis. |
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