| 赵永良,刘国廉,吴德昌,等.PCR-SSCP技术检测辐射诱发的恶转细胞p53突变[J].中华放射医学与防护杂志,1998,18(2):104-105.Zhao Yongliang,Liu Guolian,Wu Dechang,et al.Analysis of p53 mutations in radiation induced transformed RTE cells by PCR SSCP[J].Chin J Radiol Med Prot,1998,18(2):104-105 |
| PCR-SSCP技术检测辐射诱发的恶转细胞p53突变 |
| Analysis of p53 mutations in radiation induced transformed RTE cells by PCR SSCP |
| 投稿时间：1996-10-29 修订日期：1997-04-17 |
| DOI： |
| 中文关键词: 辐射 转化细胞 p53突变 |
| 英文关键词:Radiation Transformed cells p53 mutation |
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| 中文摘要: |
| 目的 确定α粒子诱发的恶性转化大鼠气管上皮(rat tracheal epithelial,RTE)细胞中是否存在p53基因突变。方法 根据大鼠p53cDNA序列, 合成4对引物分别覆盖大鼠p53基因外显子5,6,7和8,利用聚合酶链反应-单链构象多态方法分析转化细胞中的p53突变情况。结果 在转化细胞, p53基因外显子8的单链条带与正常细胞相比出现泳动变位, 经测序证实为265密码子G→C突变, 使其编码氨基酸由精氨酸→脯氨酸。结论 在α粒子诱发的恶性转化RTE细胞中存在p53基因突变, 提示p53基因变异在辐射致癌过程中可能起重要作用。 |
| 英文摘要: |
| Objective To determine whether the p53 gene has mutations in transformed RTE cells induced by α particle irradiation.Methods Four pairs of oligonucleotide primers corresponding to exon 5-8 of rat p53 gene were synthesized.The PCR single strand conformation polymorphism(SSCP) analysis was used to prescreen the p53 gene mutations.Results The single strand of exon 8 had mobility shift in polycrylamide nondenaturing gel in transformed RTE cells in contrast to normal cells.DNA sequencing proved the mutation to be G→C transversion at codon 265.Conclusions The mutation of p53 gene was found in transformed RTE cells,suggesting that the change of p53 gene might play an important role in radiation carcinogenesis. |
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