| 宋良文,赵修南,杨瑞彪,等.血管紧张素Ⅱ在60Co照射细胞增生和胶原合成中的作用[J].中华放射医学与防护杂志,1998,18(2):98-100.Song Liangwen,Zhao Xiunan,Yang Ruibiao,et al.Effect of angiotensin Ⅱ on cell proliferation and collagen synthesis following 60 Co irradiation[J].Chin J Radiol Med Prot,1998,18(2):98-100 |
| 血管紧张素Ⅱ在60Co照射细胞增生和胶原合成中的作用 |
| Effect of angiotensin Ⅱ on cell proliferation and collagen synthesis following 60 Co irradiation |
| 投稿时间：1996-09-17 修订日期：1996-12-13 |
| DOI： |
| 中文关键词: 60Co照射 成纤维细胞 增生 Ⅰ型胶原 血管紧张素Ⅱ |
| 英文关键词:60Co Irradiation Fibroblasts Proliferation Type Ⅰ collagen Angiotensin Ⅱ |
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| 中文摘要: |
| 目的 探讨60Coγ射线照射对成纤维细胞增生和Ⅰ型胶原合成的作用机制。方法 将人胚肺成纤维细胞(HELF)以不同剂量照射和用血管紧张素Ⅱ(AⅡ)刺激, 测定培养液中丙二醛含量, 检测细胞增生程度, 用免疫细胞化学显示AⅡ和Ⅰ型胶原的合成。结果 1～5Gy照射能刺激细胞增生和Ⅰ型胶原合成, 同时促进胞内AⅡ合成；外源性AⅡ能促进细胞增生及胶原合成。结论 HELF经60Co照射能过多合成AⅡ, 其可能与照射后细胞增生有关。 |
| 英文摘要: |
| Objective To explore the mechanism of 60 Co irradiation on cellular proliferation and synthesis of type Ⅰ pro-collagen protein in cultured human embryo lung fibroblasts(HELF).Method Cultured cells were divided into groups irradiated with different doses plus angiotensin Ⅱ(AⅡ) stimulation.Relationship of malondialdehyde(MDA) content in medium and extent of cellular proliferation were measured,immuno reactive material of intracellular A Ⅱ and type Ⅰ pro-collagen protein were displayed by method of immunocytochemistry,and image pattern analysis was made.Results Irradiation with 1—5Gy could stimulate cell proliferation and synthesis of type Ⅰ pro-collagen significantly;cell proliferation and type Ⅰ pro-collagen synthesis could be promoted by exogenous A Ⅱ.Conclusion Irradition can stimulate HELF to synthesize A Ⅱ,which stimulates cell proliferation and type Ⅰ pro-collagen synthesis.It may be related to HELF proliferation and-collagen synthesis induced by irradition. |
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